Liquid-Crystal Biosensor Based on Nickel-Nanosphere-Induced Homeotropic Alignment for the Amplified Detection of Thrombin

被引：63

作者：

Zhao, Dongyu ^{[1
]}

Peng, Yi ^{[1
]}

Xu, Lihong ^{[1
]}

Zhou, Wei ^{[1
]}

Wang, Qian ^{[1
]}

Guo, Lin ^{[1
]}

机构：

[1] Beijing Univ Aeronaut & Astronaut, Sch Chem & Environm Sci, Beijing 100191, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2015年 / 7卷 / 42期

基金：

中国国家自然科学基金;

关键词：

liquid crystal; thrombin; nickel nanoparticles; biosensor; signal enhancement; ELECTROCHEMICAL DETECTION; PHOSPHOLIPID MONOLAYER; DNA HYBRIDIZATION; NANOPARTICLES; AMPLIFICATION; BINDING; POLYELECTROLYTE; TRANSITIONS; INHIBITOR; SURFACES;

D O I：

10.1021/acsami.5b08924

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A new liquid-crystal (LC)-based sensor operated by nickel nanosphere (NiNS)-induced homeotropic alignment for the label-free monitoring of thrombin was reported. When doped with NiNSs, a uniform vertical orientation of 4-cyano-4'-pentylbiphenyl (5CB) was easily obtained. A sandwich system of aptamer/thrombin/aptamer-functionalized gold nanoparticles (AuNPs) was fabricated, and AuNPs aptamer conjugation caused the disruption of the 5CB orientation, leading to an obvious change of the optical appearance from a dark to a bright response to thrombin concentrations from 0.1 to 100 nM. This design also allowed quantitative detection of the thrombin concentration. This distinctive and sensitive thrombin LC sensor provides a new principle for building LC-sensing systems.

引用

页码：23418 / 23422

页数：5

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