Controlled photopolymerization of hydrogel microstructures inside microchannels for bioassays

被引：40

作者：

Liu, Jiangjiang ^{[1
]}

Gao, Dan ^{[1
]}

Li, Hai-Fang ^{[1
]}

Lin, Jin-Ming ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

来源：

LAB ON A CHIP | 2009年 / 9卷 / 09期

基金：

中国国家自然科学基金;

关键词：

MICROFLUIDIC SYSTEMS; FLOW LITHOGRAPHY; FABRICATION; PHOTOLITHOGRAPHY; MICROREACTORS; MICROARRAYS; MICROSCOPE; CHIP;

D O I：

10.1039/b819219g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes a photolithography approach to generate hydrogel microstructures inside microchannels on the basis of controlled photopolymerization of poly(ethylene glycol) diacrylate precursor by using a fluorescence microscope. The controlled photopolymerization offers: (1) morphology control on hydrogel microstructures with different illumination conditions; (2) position control on hydrogel microstructures to make it possible for the generation of arrayed hydrogel microstructures and encapsulation of single cells; (3) a convenient approach for immobilization of a variety of materials onto the solid substrates for bioassays. For demonstration, we performed a microfluidic assay for DNA oligomer detection on the arrayed hydrogel microstructures.

引用

页码：1301 / 1305

页数：5

共 25 条

[1] Photo- and electropatterning of hydrogel-encapsulated living cell arrays [J].