pH-sensor properties of a fluorescent protein from Dendronephthya sp.

被引：9
|
作者：
Pakhomov, A. A. [1 ]
Chertkova, R. V. [1 ]
Martynov, V. I. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2015年 / 41卷 / 06期
基金：
俄罗斯科学基金会;
关键词：
pH-sensor; fluorescent proteins; chromophore; chromopeptide; fluorescence; TO-RED CONVERSION; CHROMOPHORE; GFP;
D O I：
10.1134/S1068162015060114
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Genetically encoded fluorescent protein-based biosensors are widely used for pH monitoring in live cells. In this work, we have shown that fluorescent protein from Dendronephthya sp. (DendFP) has pronounced pH sensitivity. In contrast to the majority of the known genetically encoded pH-sensors, for this protein, the acidification of the environment does not lead to fluorescence quenching but just shifts it emission maximum from red to green. For this reason, it appears possible to quantitatively measure pH by the ratio of emission intensity in the red and green range, which sets DendFP apart from other pH-sensors.
引用
收藏
页码：602 / 606
页数：5
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