Enhanced Steroid Metabolites Production by Resting Cell Phytosterol Bioconversion

被引：21
作者：
Gao, X. -Q. [1 ]
Feng, J. -X. [1 ]
Wang, X. -D. [1 ]
Hua, Q. [1 ]
Wei, D. -Z. [1 ]
机构：
[1] E China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY | 2015年 / 29卷 / 04期
基金：
中国国家自然科学基金;
关键词：
phytosterol; steroid metabolites; resting cell bioconversion; hydroxypropyl-beta-cyclodextrin; optimization; MYCOBACTERIUM NEOAURUM; BIOTRANSFORMATION; DEGRADATION; CHOLESTEROL; CONVERSION; CATABOLISM;
D O I：
10.15255/CABEQ.2014.2098
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The steroid metabolites 9-hydroxy-androstenedione (9-OH-AD), androstadienedione (ADD) and androstenedione (AD) are important steroidal pharmaceuticals. In order to raise the production of steroid metabolites, an efficient resting cell phytosterol biocon-version process was developed to produce 9-OH-AD in the presence of hydroxypropyl-beta-cyclodextrin (HP-beta-CD). Cell growth medium containing phytosterol as an inducer positively improved cell activity. Under aerobic conditions, bioconversion proceeded at 70 g L-1 phytosterol in the presence of HP-beta-CD (the optimized molar ratio of - HP-beta-CD/phytosterol was 1: 1) with 30 g L-1 resting Mycobacterium neoaurum NwIB-yV cells (cell dry mass) in a 5-L bioreactor, where 9-OH-AD production and space-time yield reached 36.4 g L-1 and 9.1 g L-1 d(-1), respectively. The recycling of cells and HP-beta-CD enables cost-saving and industrial applications. This bioprocess was also applied for the production of ADD and AD. The production of these steroid metabolites was much higher than that reported in previous studies.
引用
收藏
页码：567 / 573
页数：7
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