Proteomic profile of vitreous in patients with tubercular uveitis

被引：12

作者：

Bansal, Reema ^{[1
]}

Khan, Mohd M. ^{[2
,3
,9
]}

Dasari, Surendra ^{[4
]}

Verma, Indu ^{[5
]}

Goodlett, David R. ^{[6
,10
]}

Manes, Nathan P. ^{[7
]}

Nita-Lazar, Aleksandra ^{[7
]}

Sharma, Surya P. ^{[1
]}

Kumar, Aman ^{[1
]}

Singh, Nirbhai ^{[1
]}

Chakraborti, Anuradha ^{[8
]}

Gupta, Vishali ^{[1
]}

Dogra, M. R. ^{[1
]}

Ram, Jagat ^{[1
]}

Gupta, Amod ^{[1
]}

机构：

[1] Post Grad Inst Med Educ & Res, Adv Eye Ctr, Chandigarh 160012, India

[2] Univ Maryland, Sch Med, Baltimore, MD 21201 USA

[3] NIAID, Lab Immune Syst Biol LISB, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA

[4] Mayo Clin, Dept Hlth Sci Res, Rochester, MN USA

[5] Post Grad Inst Med Educ & Res, Dept Biochem, Chandigarh, India

[6] Univ Maryland, Baltimore, MD 21201 USA

[7] NIAID, Lab Immune Syst Biol, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA

[8] Post Grad Inst Med Educ & Res, Dept Expt Med & Biotechnol, Chandigarh, India

[9] Booz Allen Hamilton, Mclean, VA USA

[10] Univ Gdansk, Gdansk, Poland

来源：

TUBERCULOSIS | 2021年 / 126卷

关键词：

Tubercular uveitis; Tuberculosis; Uveitis; Extrapulmonary tuberculosis; Shotgun proteomics; Vitreous; PROLACTIN-INDUCED PROTEIN; MYCOBACTERIUM-TUBERCULOSIS; SAMPLE PREPARATION; COMPUTATIONAL PLATFORM; AXONAL REGENERATION; ALPHA-DYSTROGLYCAN; GENE-EXPRESSION; IDENTIFICATION; DIGESTION; INJURY;

D O I：

10.1016/j.tube.2020.102036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Objective: To elucidate disease-specific host protein profile in vitreous fluid of patients with intraocular inflammation due to tubercular uveitis (TBU). Methods: Vitreous samples from 13 patients with TBU (group A), 7 with non-TBU (group B) and 9 with no uveitis (group C) were analysed by shotgun proteomics using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Differentially expressed proteins (DEPs) were subjected to pathway analysis using WEB-based Gene SeT Analysis Toolkit software. Results: Compared to control groups (B + C combined), group A (TBU) displayed 32 (11 upregulated, 21 downregulated) DEPs, which revealed an upregulation of coagulation cascades, complement and classic pathways, and downregulation of metabolism of carbohydrates, gluconeogenesis, glucose metabolism and glycolysis/gluconeogenesis pathways. When compared to group B (non-TBU) alone, TBU displayed 58 DEPs (21 upregulated, 37 downregulated), with an upregulation of apoptosis, KRAS signaling, diabetes pathways, classic pathways, and downregulation of MTORC1 signaling, glycolysis/gluconeogenesis, and glucose metabolism. Conclusion: This differential protein profile provides novel insights into the molecular mechanisms of TBU and a baseline to explore vitreous biomarkers to differentiate TBU from non-TBU, warranting future studies to identify and validate them as a diagnostic tool in TBU. The enriched pathways generate interesting hypotheses and drive further research.

引用

页数：15

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