Proteomic profile of vitreous in patients with tubercular uveitis

被引：12

作者：

Bansal, Reema ^{[1
]}

Khan, Mohd M. ^{[2
,3
,9
]}

Dasari, Surendra ^{[4
]}

Verma, Indu ^{[5
]}

Goodlett, David R. ^{[6
,10
]}

Manes, Nathan P. ^{[7
]}

Nita-Lazar, Aleksandra ^{[7
]}

Sharma, Surya P. ^{[1
]}

Kumar, Aman ^{[1
]}

Singh, Nirbhai ^{[1
]}

Chakraborti, Anuradha ^{[8
]}

Gupta, Vishali ^{[1
]}

Dogra, M. R. ^{[1
]}

Ram, Jagat ^{[1
]}

Gupta, Amod ^{[1
]}

机构：

[1] Post Grad Inst Med Educ & Res, Adv Eye Ctr, Chandigarh 160012, India

[2] Univ Maryland, Sch Med, Baltimore, MD 21201 USA

[3] NIAID, Lab Immune Syst Biol LISB, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA

[4] Mayo Clin, Dept Hlth Sci Res, Rochester, MN USA

[5] Post Grad Inst Med Educ & Res, Dept Biochem, Chandigarh, India

[6] Univ Maryland, Baltimore, MD 21201 USA

[7] NIAID, Lab Immune Syst Biol, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA

[8] Post Grad Inst Med Educ & Res, Dept Expt Med & Biotechnol, Chandigarh, India

[9] Booz Allen Hamilton, Mclean, VA USA

[10] Univ Gdansk, Gdansk, Poland

来源：

TUBERCULOSIS | 2021年 / 126卷

关键词：

Tubercular uveitis; Tuberculosis; Uveitis; Extrapulmonary tuberculosis; Shotgun proteomics; Vitreous; PROLACTIN-INDUCED PROTEIN; MYCOBACTERIUM-TUBERCULOSIS; SAMPLE PREPARATION; COMPUTATIONAL PLATFORM; AXONAL REGENERATION; ALPHA-DYSTROGLYCAN; GENE-EXPRESSION; IDENTIFICATION; DIGESTION; INJURY;

D O I：

10.1016/j.tube.2020.102036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Objective: To elucidate disease-specific host protein profile in vitreous fluid of patients with intraocular inflammation due to tubercular uveitis (TBU). Methods: Vitreous samples from 13 patients with TBU (group A), 7 with non-TBU (group B) and 9 with no uveitis (group C) were analysed by shotgun proteomics using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Differentially expressed proteins (DEPs) were subjected to pathway analysis using WEB-based Gene SeT Analysis Toolkit software. Results: Compared to control groups (B + C combined), group A (TBU) displayed 32 (11 upregulated, 21 downregulated) DEPs, which revealed an upregulation of coagulation cascades, complement and classic pathways, and downregulation of metabolism of carbohydrates, gluconeogenesis, glucose metabolism and glycolysis/gluconeogenesis pathways. When compared to group B (non-TBU) alone, TBU displayed 58 DEPs (21 upregulated, 37 downregulated), with an upregulation of apoptosis, KRAS signaling, diabetes pathways, classic pathways, and downregulation of MTORC1 signaling, glycolysis/gluconeogenesis, and glucose metabolism. Conclusion: This differential protein profile provides novel insights into the molecular mechanisms of TBU and a baseline to explore vitreous biomarkers to differentiate TBU from non-TBU, warranting future studies to identify and validate them as a diagnostic tool in TBU. The enriched pathways generate interesting hypotheses and drive further research.

引用

页数：15

共 104 条

[1] Intravitreal injection of β-crystallin B2 improves retinal ganglion cell survival in an experimental animal model of glaucoma [J].

Anders, Fabian ;

Teister, Julia ;

Liu, Aiwei ;

Funke, Sebastian ;

Grus, Franz H. ;

Thanos, Solon ;

von Pein, Harald D. ;

Pfeiffer, Norbert ;

Prokosch, Verena .

PLOS ONE, 2017, 12 (04)

[2] Autophagy induction targeting mTORC1 enhances Mycobacterium tuberculosis replication in HIV co-infected human macrophages [J].

Andersson, Anna-Maria ;

Andersson, Blanka ;

Lorell, Christoffer ;

Raffetseder, Johanna ;

Larsson, Marie ;

Blomgran, Robert .

SCIENTIFIC REPORTS, 2016, 6

[3]

Angeles-Han S, 2016, ARTHRITIS RHEUMATOL, V68

[4]

[Anonymous], 2013, PLOS ONE, DOI DOI 10.1371/journal.pone.0056540

[5]

Apweiler R, 2004, NUCLEIC ACIDS RES, V32, pD115, DOI [10.1093/nar/gkw1099, 10.1093/nar/gkh131]

[6] Levels of complement C3 and C4 components in Amerindians living in an area with high prevalence of tuberculosis [J].

Araujo, Zaida ;

Gonzalez, Nieves ;

de Cubeddu, Laura ;

Ziegler, Rita C. ;

de Waard, Jacobus H. ;

Giampietro, Francesca ;

Garzaro, Domingo ;

Pujol, Flor H. ;

Carrasco de Serrano, Neligia ;

Garcia de Saboin, Amairany .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2006, 101 (04) :359-364

[7] Analytical Exploration of Potential Pathways by which Diabetes Mellitus Impacts Tuberculosis Epidemiology [J].

Awad, Susanne F. ;

Dargham, Soha R. ;

Omori, Ryosuke ;

Pearson, Fiona ;

Critchley, Julia A. ;

Abu-Raddad, Laith J. .

SCIENTIFIC REPORTS, 2019, 9 (1)

[8] Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis [J].

Ayuso, Viera Kalinina ;

de Boer, Joke H. ;

Byers, Helen L. ;

Coulton, Gary R. ;

Dekkers, Jojanneke ;

de Visser, Lenneke ;

van Loon, Anton M. ;

Schellekens, Peter A. W. J. F. ;

Rothova, Aniki ;

de Groot-Mijnes, Jolanda D. F. .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2013, 54 (05) :3709-3720

[9] Apoptosis is an innate defense function of macrophages against Mycobacterium tuberculosis [J].

Behar, S. M. ;

Martin, C. J. ;

Booty, M. G. ;

Nishimura, T. ;

Zhao, X. ;

Gan, H. ;

Divangahi, M. ;

Remold, H. G. .

MUCOSAL IMMUNOLOGY, 2011, 4 (03) :279-287

[10] Mediators and biomarkers of inflammation in meningitis: Cytokine and peptidome profiling of cerebrospinal fluid [J].

Belogurov, A. A., Jr. ;

Ivanova, O. M. ;

Lomakin, Y. A. ;

Ziganshin, R. H. ;

Vaskina, M. I. ;

Knorre, V. D. ;

Klimova, E. A. ;

Gabibov, A. G. ;

Ivanov, V. T. ;

Govorun, V. M. .

BIOCHEMISTRY-MOSCOW, 2016, 81 (11) :1293-1302

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