Concentrations of the major birch tree allergen Bet v 1 in pollen and respirable fine particles in the atmosphere

被引:132
|
作者
Schappi, GF [1 ]
Suphioglu, C [1 ]
Taylor, PE [1 ]
Knox, RB [1 ]
机构
[1] UNIV MELBOURNE,SCH BOT,POLLEN & ALLERGEN RES GRP,PARKVILLE,VIC 3052,AUSTRALIA
基金
澳大利亚研究理事会; 英国医学研究理事会;
关键词
Bet v 1; birch pollen; respirable fine particles; birch pollen equivalents; allergen; asthma;
D O I
10.1016/S0091-6749(97)70170-2
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Birch tree pollen allergens are an important cause of early spring hay fever and allergic asthma. Pollen counts provide a guide for individuals with birch pollen allergy. However, birch pollen, because of its size, has a low probability of entering the lower airways to trigger asthma, Yet birch pollen allergens are known to be associated with respirable particles present in the atmosphere. Objective: We sought to determine the concentration of major allergen Bet v 1 in birch pollen and respirable particles in the atmosphere during the birch pollen season. Methods: We used a two-site monoclonal antibody-based assay (ELISA) to quantitate Bet v 1 in pollen extracts and high-volume air sampler filters collecting particles larger and smaller than 7.2 mu m. Results: Bet v 1 (0.006 ng) is detectable per birch pollen grain, of which 0.004 ng is present in aqueous extracts (13.9% of soluble proteins), Atmospheric Bet v 1 concentrations are correlated with birch pollen counts, Heavy rainfall tended to wash out pollen and particles, indicated by a mean daily Bet v 1 concentration of 0.12 ng/m(3) (20 pollen equivalents), but light rainfall produced a dramatic increase in allergen-loaded respirable particles with Bet vl concentrations of 1.2 ng/m(3) (200 pollen equivalents). Conclusion: These results highlight the different environmental risk factors for hay fever and allergic asthma in patients sensitized to Bet v 1. Light rainfall causes an increase in respirable particles; hence, this is an important risk factor for asthma.
引用
收藏
页码:656 / 661
页数:6
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