A novel hamster nonalcoholic steatohepatitis model induced by a high-fat and high-cholesterol diet

被引：9

作者：

Miyaoka, Yuta ^{[1
]}

Jin, Denan ^{[2
]}

Tashiro, Keitaro ^{[1
]}

Masubuchi, Shinsuke ^{[1
]}

Ozeki, Maiko ^{[1
]}

Hirokawa, Fumitoshi ^{[1
]}

Hayashi, Michihiro ^{[1
]}

Takai, Shinji ^{[2
]}

Uchiyama, Kazuhisa ^{[1
]}

机构：

[1] Osaka Med Coll, Dept Gen & Gastroenterol Surg, 2-7 Daigaku Machi, Takatsuki, Osaka 5698686, Japan

[2] Osaka Med Coll, Grad Sch Med, Dept Innovat Med, 2-7 Daigaku Machi, Takatsuki, Osaka 5698686, Japan

来源：

EXPERIMENTAL ANIMALS | 2018年 / 67卷 / 02期

基金：

日本学术振兴会;

关键词：

chymase; fibrosis; hamster; nonalcoholic steatohepatitis; steatosis; CHYMASE INHIBITOR PREVENTS; CHOLINE-DEFICIENT DIET; LIVER-DISEASE; MAST-CELLS; RAT MODEL; FIBROSIS; PROGRESSION; ACTIVATION; SURVIVAL; THERAPY;

D O I：

10.1538/expanim.17-0126

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Nonalcoholic steatohepatitis (NASH), in which there is steatosis and fibrosis in the liver, is linked to metabolic syndrome and progresses to hepatic cirrhosis. In this study, a novel hamster NASH model derived from metabolic syndrome was made using hamsters. Hamsters were fed a normal or a high-fat and high-cholesterol (HFC) diet for 12 weeks. Body weight and the ratio of liver weight to body weight were significantly greater in HFC diet-fed hamsters than in normal diet-fed hamsters. Triglyceride, low-density lipoprotein cholesterol, and glucose levels in blood were significantly increased in HFC diet-fed hamsters, and blood pressure also tended to be high, suggesting that the HFC diet-fed hamsters developed metabolic syndrome. Hepatic steatosis and fibrosis were observed in liver sections of HFC diet-fed hamsters, as in patients with NASH, but they were not seen in normal diet-fed hamsters. Chymase generates angiotensin II and transforming growth factor (TGF)-beta, both of which are related to hepatic steatosis and fibrosis, and a significant augmentation of chymase activity was observed in livers from HFC diet-fed hamsters. Both angiotensin II and TGF-beta were also significantly increased in livers of HFC diet-fed hamsters. Thus, HFC diet-fed hamsters might develop metabolic syndrome-derived NASH that clinically resembles that in NASH patients.

引用

页码：239 / 247

页数：9

共 22 条

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