Development and characterization of six new microsatellite markers for the silver- or gold-lipped pearl oyster, Pinctada maxima (Pteriidae)

被引：18
作者：
Evans, B. S. [1 ]
Knauer, J.
Taylor, J. J. U.
Jerry, D. R.
机构：
[1] James Cook Univ N Queensland, Aquaculture Genet Res Grp, Sch Marine Biol & Aquaculture, Townsville, Qld 4811, Australia
[2] Atlas Pacific Ltd, PT Cendana Indopearls, Denapasar, Indonesia
来源：
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 03期
关键词：
DNA pedigree; enrichment; parentage; pearl culture; population genetics;
D O I：
10.1111/j.1471-8286.2006.01362.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Six di-, tri- and tetranucleotide microsatellite loci were developed for the silver- or gold-lipped pearl oyster Pinctada maxima using a linker-ligated, magnetic bead enrichment protocol. Based on a minimum of 134 Indonesian pearl oyster samples, number of alleles and observed heterozygosity at each locus ranged from six to 17 alleles and from 0.172 to 0.813 (mean = 0.448), respectively. Mean polymorphic information content for the six loci was 0.562. These loci should be very useful in DNA parentage analyses and population differentiation of P. maxima in Australia and Indonesia.
引用
收藏
页码：835 / 837
页数：3
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