CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera

被引：39

作者：

Cheng, Yang ^{[1
,2
]}

Lin, Zhe ^{[1
]}

Wang, Ju-Mei ^{[1
]}

Xing, Long-Sheng ^{[1
]}

Xiong, Guang-Hua ^{[1
,3
]}

Zou, Zhen ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Jinggangshan Univ, Sch Life Sci, Jian 343009, Jiangxi, Peoples R China

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2018年 / 84卷

基金：

美国国家科学基金会;

关键词：

Helicoverpa armigera; Entomopathogen; Beauveria bassiana; C-type lectin; Anti-Fungal immunity; C-TYPE LECTIN; INSECT INNATE IMMUNITY; ACUTE-PHASE PROTEIN; MANDUCA-SEXTA; AEDES-AEGYPTI; DROSOPHILA-MELANOGASTER; ENTOMOPATHOGENIC FUNGI; CELLULAR ENCAPSULATION; MELANIZATION; ACTIVATION;

D O I：

10.1016/j.dci.2018.02.010

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was found to form aggregates with zymosan and B. bassiana in vitro. lmmunoprecipitation studies demonstrated that CTL14 interacted with serine proteinases (SP), serine proteinase inhibitor (serpin), prophenoloxidases (PPO) and vitellogenin (Vg) in the larval hemolymph. Furthermore, depletion of CTL14 using dsRNA led to dramatic decrease in the expression level of PPO1. Additionally, CTL14 depleted H. armigera decreased the resistance to fungal challenge. Taken together, our study showed the direct involvement of CTL14 in the anti-fungal immunity of H. armigera, which further explained the stronger immune responses in the fifth instar compared to the second instar larvae. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：142 / 152

页数：11

共 54 条

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