Clostridium manihotivorum sp. nov., a novel mesophilic anaerobic bacterium that produces cassava pulp-degrading enzymes

被引：8

作者：

Cheawchanlertfa, Pattsarun ^{[1
]}

Sutheeworapong, Sawannee ^{[2
]}

Jenjaroenpun, Piroon ^{[3
,4
]}

Wongsurawat, Thidathip ^{[3
,4
]}

Nookaew, Intawat ^{[4
,5
]}

Cheevadhanarak, Supapon ^{[1
,2
]}

Kosugi, Akihiko ^{[6
]}

Pason, Patthra ^{[1
,2
]}

Waeonukul, Rattiya ^{[1
,2
]}

Ratanakhanokchai, Khanok ^{[1
]}

Tachaapaikoon, Chakrit ^{[1
,2
]}

机构：

[1] King Mongkuts Univ Technol Thonburi, Sch Bioresources & Technol, Bangkok, Thailand

[2] King Mongkuts Univ Technol Thonburi, Pilot Plant Dev & Training Inst, Bangkok, Thailand

[3] Mahidol Univ, Siriraj Hosp, Fac Med, Div Bioinformat & Data Management Res,Dept Res &, Bangkok, Thailand

[4] Univ Arkansas Med Sci, Coll Med, Dept Biomed Informat, Little Rock, AR 72205 USA

[5] Univ Arkansas Med Sci, Coll Med, Dept Physiol & Biophys, Little Rock, AR 72205 USA

[6] Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Ibaraki, Japan

来源：

PEERJ | 2020年 / 8卷

基金：

美国国家卫生研究院;

关键词：

Cassava pulp-degrading enzymes; Clostridium species; Complete genome sequence; Illumina; Mesophilic anaerobic bacterium; Oxford nanopore technology; SACCHARIFICATION; CELL; FERMENTATION; CELLULOSOME; CELLULASE; TAXONOMY; XYLANASE; ANNOTATION; SUBSTRATE; BUTYRICUM;

D O I：

10.7717/peerj.10343

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background. Cassava pulp is a promising starch-based biomasses, which consists of residual starch granules entrapped in plant cell wall containing non-starch polysaccharides, cellulose and hemicellulose. Strain CT4(T), a novel mesophilic anaerobic bacterium isolated from soil collected from a cassava pulp landfill, has a strong ability to degrade polysaccharides in cassava pulp. This study explored a rarely described species within the genus Clostridium that possessed a group of cassava pulp-degrading enzymes. Methods. A novel mesophilic anaerobic bacterium, the strain CT4(T), was identified based on phylogenetic, genomic, phenotypic and chemotaxonomic analysis. The complete genome of the strain CT4(T) was obtained following whole-genome sequencing, assembly and annotation using both Illumina and Oxford Nanopore Technology (ONT) platforms. Results. Analysis based on the 16S rRNA gene sequence indicated that strain CT4(T) is a species of genus Clostridium. Analysis of the whole-genome average amino acid identity (AAI) of strain CT4(T) and the other 665 closely related species of the genus Clostridium revealed a separated strain CT4(T) from the others. The results revealed that the genome consisted of a 6.3 Mb circular chromosome with 5,664 protein-coding sequences. Genome analysis result of strain CT4(T) revealed that it contained a set of genes encoding amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. A comparative genomic analysis of strain CT4(T) with closely related species with available genomic information, C. amylolyticum SW408(T), showed that strain CT4(T) contained more genes encoding cassava pulp-degrading enzymes, which comprised a complex mixture of amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. This work presents the potential for saccharification of strain CT4(T) in the utilization of cassava pulp. Based on phylogenetic, genomic, phenotypic and chemotaxonomic data, we propose a novel species for which the name Clostridium manihotivorum sp. nov. is suggested, with the type strain CT4(T) (= TBRC 11758(T) = NBRC 114534(T) ).

引用

页数：28

共 76 条

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