SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand

被引：22

作者：

Choi, Jae Min ^{[1
]}

Hahm, Eunil ^{[2
]}

Park, Kyeonghui ^{[3
,4
]}

Jeong, Daham ^{[3
,4
]}

Rho, Won-Yeop ^{[2
]}

Kim, Jaehi ^{[5
]}

Jeong, Dae Hong ^{[5
]}

Lee, Yoon-Sik ^{[6
]}

Jhang, Sung Ho ^{[7
]}

Chung, Hyun Jong ^{[7
]}

Cho, Eunae ^{[1
]}

Yu, Jae-Hyuk ^{[8
,9
]}

Jun, Bong-Hyun ^{[2
]}

Jung, Seunho ^{[1
,3
,4
]}

机构：

[1] Konkuk Univ, Inst Ubiquitous Informat Technol & Applicat UBITA, Ctr Biotechnol Res UBITA CBRU, Seoul 05029, South Korea

[2] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South Korea

[3] Konkuk Univ, Dept Biosci & Biotechnol, MCRB, Seoul 05029, South Korea

[4] Konkuk Univ, Ctr Biotechnol Res UBITA CBRU, Seoul 05029, South Korea

[5] Seoul Natl Univ, Dept Chem Educ, Seoul 08826, South Korea

[6] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 08826, South Korea

[7] Konkuk Univ, Dept Phys, Seoul 05029, South Korea

[8] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

[9] Univ Wisconsin, Dept Genet, Madison, WI 53706 USA

来源：

NANOMATERIALS | 2017年 / 7卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

cyclodextrin; ethylenediamine cyclodextrin; surface-enhanced Raman scattering (SERS); flavonoids; ENHANCED RAMAN-SCATTERING; INCLUSION COMPLEX; ORGANIC-COMPOUNDS; SOLID-PHASE; QUERCETIN; NANOPARTICLES; SOLUBILITY; LUTEOLIN; ANTIOXIDANT; PREVENTION;

D O I：

10.3390/nano7010008

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ethylenediamine-modified -cyclodextrin (Et-beta-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO2@Ag@Et--CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-beta-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO2@Ag@Et-beta-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin. In particular, luteolin was detected more strongly in the linear range 10(-7) to 10(-3) M than various organic molecules, namely ethylene glycol, beta-estradiol, isopropyl alcohol, naphthalene, and toluene. In addition, the SERS signal for luteolin captured by the SiO2@Ag@Et-beta-CD NPs remained even after repeated washing. These results indicated that the SiO2@Ag@Et-beta-CD NPs can be used as a rapid, sensitive, and selective sensor for flavonoids.

引用

页数：12

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[1] Phase solubility and inclusion complex of itraconazole with β-cyclodextrin using supercritical carbon dioxide [J].