Wide geographical dissemination of the multiresistant Staphylococcus capitis NRCS-A clone in neonatal intensive-care units

被引：49

作者：

Butin, M. ^{[1
,2
]}

Rasigade, J. -P. ^{[1
,2
,3
,4
]}

Martins-Simoes, P. ^{[1
,2
,3
]}

Meugnier, H. ^{[2
,3
]}

Lemriss, H. ^{[5
]}

Goering, R. V. ^{[6
]}

Kearns, A. ^{[7
]}

Deighton, M. A. ^{[8
]}

Denis, O. ^{[9
]}

Ibrahimi, A. ^{[5
]}

Claris, O. ^{[4
,10
]}

Vandenesch, F. ^{[1
,2
,3
,4
]}

Picaud, J. -C. ^{[4
,11
]}

Laurent, F. ^{[1
,2
,3
,4
]}

机构：

[1] Hosp Civils Lyon, Northern Hosp Grp, Dept Clin Microbiol, Lyon, France

[2] Univ Lyon 1, CNRS UMR5308, Int Ctr Res Infect Dis CIRI, INSERM U1111,ENS Lyon, F-69365 Lyon, France

[3] Hosp Civils Lyon, Natl Reference Ctr Staphylococci, Lyon, France

[4] Univ Lyon 1, F-69622 Villeurbanne, France

[5] Mohamed V Univ Rabat, Fac Med & Pharm Rabat, Biotechnol Lab, Rabat, Morocco

[6] Creighton Univ, Omaha, NE 68178 USA

[7] Publ Hlth England, Staphylococcus Reference Serv, London, England

[8] RMIT Univ, Bundoora, Vic, Australia

[9] Univ Libre Bruxelles, Erasme Hosp, Lab Reference MRSA, Staphylocoques,Dept Microbiol, Brussels, Belgium

[10] Hosp Civils Lyon, Eastern Hosp Grp, Neonatal Intens Care Unit, Bron, France

[11] Hosp Civils Lyon, Northern Hosp Grp, Neonatal Intens Care Unit, Lyon, France

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2016年 / 22卷 / 01期

关键词：

Antibiotic resistance; clone; coagulase-negative Staphylococcus; neonatology; vancomycin; FIELD GEL-ELECTROPHORESIS; LATE-ONSET SEPSIS; VANCOMYCIN; HETERORESISTANCE; PATTERNS; INFANTS; AUREUS; STRAIN; MEC;

D O I：

10.1016/j.cmi.2015.09.008

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Nosocomial late-onset sepsis represents a frequent cause of morbidity and mortality in preterm neonates. The Staphylococcus capitis clone NRCS-A has been previously described as an emerging cause of nosocomial bacteraemia in French neonatal intensive-care units (NICUs). In this study, we aimed to explore the possible unrecognized dissemination of this clone on a larger geographical scale. One hundred methicillin-resistant S. capitis strains isolated from neonates (n = 86) and adult patients (n = 14) between 2000 and 2013 in four different countries (France, Belgium, the UK, and Australia) were analysed with SmaI pulsed-field gel electrophoresis (PFGE) and dru typing. The vast majority of NICU strains showed the NRCS-A pulsotype and the dt11c type (96%). We then randomly selected 14 isolates (from neonates, n = 12, three per country; from adult patients, n = 2), considered to be a subset of representative isolates, and performed further molecular typing (SacII PFGE, SCCmec typing, and multilocus sequence typing-like analysis), confirming the clonality of the S. capitis strains isolated from neonates, despite their distant geographical origin. Whole genome single-nucleotide polymorphism-based phylogenetic analysis of five NICU isolates (from the different countries) attested to high genetic relatedness within the NRCS-A clone. Finally, all of the NRCS-A strains showed multidrug resistance (e.g. methicillin and aminoglycoside resistance, and decreased vancomycin susceptibility), with potential therapeutic implications for infected neonates. In conclusion, this study represents the first report of clonal dissemination of methicillin-resistant coagulase-negative Staphylococcus clone on a large geographical scale. Questions remain regarding the origin and means of international spread, and the reasons for this clone's apparent predilection for neonates. (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

引用

页码：46 / 52

页数：7

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