Unveiling the anti-inflammatory activity of Sutherlandia frutescens using murine macrophages

被引：13

作者：

Lei, Wei ^{[1
]}

Browning, Jimmy D., Jr. ^{[1
]}

Eichen, Peggy A. ^{[1
]}

Brownstein, Korey J. ^{[2
]}

Folk, William R. ^{[2
]}

Sun, Grace Y. ^{[2
]}

Lubahn, Dennis B. ^{[2
]}

Rottinghaus, George E. ^{[3
]}

Fritsche, Kevin L. ^{[1
,4
]}

机构：

[1] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA

[2] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA

[3] Univ Missouri, Vet Med Diagnost Lab, Columbia, MO 65211 USA

[4] Univ Missouri, Dept Nutr & Exercise Physiol, Columbia, MO 65211 USA

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2015年 / 29卷 / 02期

关键词：

Sutherlandia frutescens; Inflammation; Macrophage; Chlorophyll; NITRIC-OXIDE PRODUCTION; TOLL-LIKE RECEPTORS; L; R; BR; IFN-GAMMA; IN-VITRO; NADPH OXIDASE; ANTIOXIDANT; FABACEAE; CHLOROPHYLLIN; CANCER;

D O I：

10.1016/j.intimp.2015.11.012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Sutherlandia frutescens is a botanical widely used in southern Africa for treatment of inflammatory and other conditions. Previously, an ethanolic extract of S. frutescens (SFE) has been shown to inhibit the production of reactive oxygen species (ROS) and nitric oxide (NO) by murine neurons and a microglia cell line (BV-2 cells). In this study we sought to confirm the anti-inflammatory activities of SFE on a widely used murine macrophage cell line (i.e., RAW 264.7 cells) and primary mouse macrophages. Furthermore, experiments were conducted to investigate the anti-inflammatory activity of the flavonol and cycloartanol glycosides found in high quantities in S. frutescens. While the SFE exhibited anti-inflammatory activities upon murine macrophages similar to that reported with the microglia cell line, this effect does not appear to be mediated by sutherlandiosides or sutherlandins. In contrast, chlorophyll in our extracts appeared to be partly responsible for some of the activity observed in our macrophage-dependent screening assay. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：254 / 262

页数：9

共 40 条

[21] Sutherlandia frutescens Ethanol Extracts Inhibit Oxidative Stress and Inflammatory Responses in Neurons and Microglial Cells [J].