Characterization of apple stem grooving virus and apple chlorotic leaf spot virus identified in a crab apple tree

被引:7
|
作者
Li, Yongqiang [1 ]
Deng, Congliang [2 ]
Bian, Yong [2 ]
Zhao, Xiaoli [2 ]
Zhou, Qi [3 ]
机构
[1] Beijing Agr Univ, Coll Plant Sci & Technol, Beijing 102206, Peoples R China
[2] Beijing Entry Exit Inspect & Quarantine Bur, Plant Lab, Beijing 101312, Peoples R China
[3] Chinese Soc Inspect & Quarantine, Beijing 100029, Peoples R China
关键词
RNA-RNA RECOMBINATION; INFECTION; SEQUENCES;
D O I
10.1007/s00705-016-3183-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and prunus necrotic ringspot virus (PNRSV) were identified in a crab apple tree by small RNA deep sequencing. The complete genome sequence of ACLSV isolate BJ (ACLSV-BJ) was 7554 nucleotides and shared 67.0%-83.0% nucleotide sequence identity with other ACLSV isolates. A phylogenetic tree based on the complete genome sequence of all available ACLSV isolates showed that ACLSV-BJ clustered with the isolates SY01 from hawthorn, MO5 from apple, and JB, KMS and YH from pear. The complete nucleotide sequence of ASGV-BJ was 6509 nucleotides (nt) long and shared 78.2%-80.7% nucleotide sequence identity with other isolates. ASGV-BJ and the isolate ASGV_ kfp clustered together in the phylogenetic tree as an independent clade. Recombination analysis showed that isolate ASGVBJ was a naturally occurring recombinant.
引用
收藏
页码:1093 / 1097
页数:5
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