共 42 条
Different mitochondrial-intermembrane space proteins are released during apoptosis in a manner that is coordinately initiated but can vary in duration
被引:196
作者:

Munoz-Pinedo, Cristina
论文数: 0 引用数: 0
h-index: 0
机构: St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA

Guio-Carrion, Ana
论文数: 0 引用数: 0
h-index: 0
机构: St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA

Goldstein, Joshua C.
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h-index: 0
机构: St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA

Fitzgerald, Patrick
论文数: 0 引用数: 0
h-index: 0
机构: St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA

Newmeyer, Donald D.
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h-index: 0
机构: St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA

Green, Douglas R.
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h-index: 0
机构: St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA
机构:
[1] St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA
[2] Hosp Barcelona, Ctr Mol Oncol, Inst Rec Oncol, IDIBELL, Barcelona 08907, Spain
[3] La Jolla Inst Allergy & Immunol, San Diego, CA 92121 USA
来源:
关键词:
apoptosis-inducing factor;
cytochrome c;
mitochondria;
Omi;
Smac;
D O I:
10.1073/pnas.0603007103
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
The release of mitochondrial intermembrane space proteins to the cytosol is a key event during apoptosis. We used in situ fluorescent labeling of proteins tagged with a short tetracysteine-containing sequence to follow the release of Smac, Omi, adenylate kinase-2, cytochrome c, and apoptosis-inducing factor (AIF) during apoptosis and compared the release with that of cytochrome c tagged with GFP in individual cells observed overtime. We observed a caspase-independent, simultaneous release of cytochrome c, Smac, Omi, and adenylate kinase-2. Although AIF release also was caspase-independent and commenced with that of the other proteins, it proceeded much more slowly and incompletely from mitochondria, perhaps because of a requirement for a secondary event. These results suggest that these proteins are released through the same mitochondrial pore and that apoptosis may not be regulated through a selective release of individual mitochondrial proteins. The timing and extent of AIF release makes it unlikely that it is involved in the induction of apoptosis, either upstream or downstream of mitochondrial outer membrane permeabilization.
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页码:11573 / 11578
页数:6
相关论文
共 42 条
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