Expression of α-hemoglobin stabilizing protein gene during human erythropoiesis

被引：47

作者：

dos Santos, CO ^{[1
]}

Duarte, ASS ^{[1
]}

Saad, STO ^{[1
]}

Costa, FF ^{[1
]}

机构：

[1] Univ Estadual Campinas, UNICAMP, Hemoctr, Ctr Hemotherapy & Hematol, BR-13083970 Campinas, SP, Brazil

来源：

EXPERIMENTAL HEMATOLOGY | 2004年 / 32卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

D O I：

10.1016/j.exphem.2003.11.002

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

alpha-Hemoglobin stabilizing protein (AHSP) is an abundant, erythroid-specific protein that forms a stable complex with free alpha-hemoglobin but not with beta-hemoglobin or hemoglobin A. As such, AHSP is required for normal erythropoiesis, probably acting by blocking the deleterious effects of free alpha-hemoglobin precipitation. In order to study the levels of expression of the AHSP gene during the different phases of erythropoiesis, we carried out a two-phase liquid culture of erythroid cells and real-time quantitative polymerase chain reaction. Blood from control volunteers was cultured with erythropoietin to stimulate differentiation. The different stages of erythropoiesis were confirmed by morphologic and flow cytometric analysis. The results showed a progressive increase in AHSP gene expression following the expression of alpha-globin gene, during maturation of the red blood cell precursors, confirming the probable important function of this protein during normal erythropoiesis. (C) 2004 International Society for Experimental Hematology. Published by Elsevier Inc.

引用

页码：157 / 162

页数：6

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