Multiplex Cytological Profiling Assay to Measure Diverse Cellular States

被引：189

作者：

Gustafsdottir, Sigrun M.

Ljosa, Vebjorn

Sokolnicki, Katherine L.

Wilson, J. Anthony

Walpita, Deepika

Kemp, Melissa M.

Seiler, Kathleen Petri

Carrel, Hyman A.

Golub, Todd R.

Schreiber, Stuart L.

Clemons, Paul A. ^{[1
]}

Carpenter, Anne E.

Shamji, Alykhan F.

机构：

[1] Broad Inst Harvard, Cambridge, MA USA

来源：

PLOS ONE | 2013年 / 8卷 / 12期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

RESPONSES; DATABASE; LIGAND; CELLS;

D O I：

10.1371/journal.pone.0080999

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound-and disease-associated cell states to support future probe discovery.

引用

页数：7

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