Survival of Escherichia coli O157:H7 in Soils from Jiangsu Province, China

被引：24

作者：

Zhang, Taoxiang ^{[1
]}

Wang, Haizhen ^{[1
]}

Wu, Laosheng ^{[1
]}

Lou, Jun ^{[1
]}

Wu, Jianjun ^{[1
]}

Brookes, Philip C. ^{[1
]}

Xu, Jianming ^{[1
]}

机构：

[1] Zhejiang Univ, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Coll Environm & Nat Resource Sci, Hangzhou 310003, Zhejiang, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 12期

基金：

中国国家自然科学基金;

关键词：

ENTERICA SEROVAR TYPHIMURIUM; MANURE-AMENDED SOIL; MICROBIAL COMMUNITIES; O157-H7; PH; RHIZOSPHERE; O157/H7; CULTIVATION; DIVERSITY; FATE;

D O I：

10.1371/journal.pone.0081178

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Escherichia coli O157:H7 (E. coli O157: H7) is recognized as a hazardous microorganism in the environment and for public health. The E. coli O157: H7 survival dynamics were investigated in 12 representative soils from Jiangsu Province, where the largest E. coli O157: H7 infection in China occurred. It was observed that E. coli O157: H7 declined rapidly in acidic soils (pH, 4.57 - 5.14) but slowly in neutral soils (pH, 6.51 - 7.39). The survival dynamics were well described by the Weibull model, with the calculated t(d) value (survival time of the culturable E. coli O157: H7 needed to reach the detection limit of 100 CFU g(-1)) from 4.57 days in an acidic soil (pH, 4.57) to 34.34 days in a neutral soil (pH, 6.77). Stepwise multiple regression analysis indicated that soil pH and soil organic carbon favored E. coli O157: H7 survival, while a high initial ratio of Gram-negative bacteria phospholipid fatty acids (PLFAs) to Gram-positive bacteria PLFAs, and high content of exchangeable potassium inhibited E. coli O157: H7 survival. Principal component analysis clearly showed that the survival profiles in soils with high pH were different from those with low pH.

引用

页数：6