Difference in lipid cell composition and shaped-based gold nanoparticles induce distinguish pathways in Langmuir monolayers response

Understanding the interactions occurring at the nano-bio interface is still a challenge. This study presents an investigation on the interactions between natural cell membranes and gold-based particles that were isolated from real - cancer and health - cells and reconstructed as monolayers. The influence of the morphology and surface charge of gold nanomaterials on the membrane structure, as well as the interface properties of the membranes was evaluated. Our results revealed that the interaction pathway of nanoparticles through the cell membrane was affected by the lipidic composition of each cell type evaluated. Moreover, we show that the surface charge of the nanoparticles plays a significant role in their ability to interact with cell membranes, as revealed by isothermal titration calorimetry, and atomic force microscopy analysis.