Identification of a soluble NADPH oxidoreductase (BmNOX) with antiviral activites in the gut juice of Bombyx mori

被引：56
作者：
Selot, Ruchita
Kumar, Virendra
Shukla, Sunita
Chandrakuntal, Kumar
Brahmaraju, Mopidevi
Dandin, S. B.
Laloraya, Malini
Kumar, Pradeep G.
机构：
[1] Rajiv Gandhi Ctr Biotechnol, Trivandrum 695014, Kerala, India
[2] Cent Sericultural Res & Training Inst, Mysore 570008, Karnataka, India
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2007年 / 71卷 / 01期
关键词：
silkworm; anti-viral; NOX; virus resistance;
D O I：
10.1271/bbb.60450
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Silkworms show high variability in silk quality and disease resistance. Attempts are on to combine the disease tolerance of multivoltine races and the silk quality of bivoltine races to generate new races with desirable phenotypic traits. We report the identification of a 26.5-kDa protein that is overexpressed in the gut juice of disease-resistant multivoltine races and that has anti-BmNPV activity. We have characterized this protein as a soluble NADH-oxidoreductase-like protein (BmNOX). Treatment of live BmNPV particles with BmNOX inhibited the capability of the viral particles to infect BmN cells in vitro.
引用
收藏
页码：200 / 205
页数：6
相关论文
共 28 条
  • [1] Superoxide production in Galleria mellonella hemocytes:: Identification of proteins homologous to the NADPH oxidase complex of human neutrophils
    Bergin, D
    Reeves, EP
    Renwick, J
    Wientjes, FB
    Kavanagh, K
    [J]. INFECTION AND IMMUNITY, 2005, 73 (07) : 4161 - 4170
  • [2] Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx mori
    Cheng, TC
    Zhao, P
    Liu, C
    Xu, PZ
    Gao, ZH
    Xia, QY
    Xiang, ZH
    [J]. GENOMICS, 2006, 87 (03) : 356 - 365
  • [3] Antiviral and antitumor peptides from insects
    Chernysh, S
    Kim, SI
    Bekker, G
    Pleskach, VA
    Filatova, NA
    Anikin, VB
    Platonov, VG
    Bulet, P
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 12628 - 12632
  • [4] Insect defenses against virus infection: The role of apoptosis
    Clarke, TE
    Clem, RJ
    [J]. INTERNATIONAL REVIEWS OF IMMUNOLOGY, 2003, 22 (5-6) : 401 - 424
  • [5] Clem RJ, 2005, CURR TOP MICROBIOL, V289, P113
  • [6] FUNCTIONAL RECONSTITUTION OF THE PHAGOCYTE NADPH OXIDASE BY TRANSFECTION OF ITS MULTIPLE COMPONENTS IN A HETEROLOGOUS SYSTEM
    DEMENDEZ, I
    LETO, TL
    [J]. BLOOD, 1995, 85 (04) : 1104 - 1110
  • [7] Roles of nonstructural protein nsP2 and alpha/beta interferons in determining the outcome of Sindbis virus infection
    Frolova, EI
    Fayzulin, RZ
    Cook, SH
    Griffin, DE
    Rice, CM
    Frolov, I
    [J]. JOURNAL OF VIROLOGY, 2002, 76 (22) : 11254 - 11264
  • [8] Glupov VV, 2001, CYTOBIOS, V106, P165
  • [9] A direct role for dual oxidase in Drosophila gut immunity
    Ha, EM
    Oh, CT
    Bae, YS
    Lee, WJ
    [J]. SCIENCE, 2005, 310 (5749) : 847 - 850
  • [10] Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens
    Kavanagh, K
    Reeves, EP
    [J]. FEMS MICROBIOLOGY REVIEWS, 2004, 28 (01) : 101 - 112
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