Establishment and Long-Term Expansion of Small Cell Lung Cancer Patient-Derived Tumor Organoids

被引：33

作者：

Choi, Seon Young ^{[1
]}

Cho, Yong-Hee ^{[1
]}

Kim, Da-Som ^{[2
]}

Ji, Wonjun ^{[3
]}

Choi, Chang-Min ^{[3
]}

Lee, Jae Cheol ^{[4
]}

Rho, Jin Kyung ^{[5
]}

Jeong, Gi Seok ^{[1
,5
,6
]}

机构：

[1] Asan Med Ctr, Asan Inst Life Sci, Seoul 05505, South Korea

[2] Univ Ulsan, Dept Biomed Sci, Asan Med Ctr, AMIST,Coll Med, Seoul 05505, South Korea

[3] Univ Ulsan, Dept Pulmonol & Crit Care Med, Coll Med, Asan Med Ctr, Seoul 05505, South Korea

[4] Univ Ulsan, Dept Oncol, Coll Med, Asan Med Ctr, Seoul 05505, South Korea

[5] Univ Ulsan, Dept Convergence Med, Coll Med, Asan Med Ctr, Seoul 05505, South Korea

[6] Asan Med Ctr, Asan Inst Life Sci, Biomed Engn Res Ctr, Seoul 05505, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

small cell lung cancer; patient-derived tumor organoids; patient avatar model systems;

D O I：

10.3390/ijms22031349

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential chemo-sensitivity of cancer cells, which is attributed to the cellular heterogeneity and phenotypic variation of cancer cells, is considered to be the main reason for tumor recurrence after chemotherapy. Here, we generated small cell lung cancer patient-derived tumor organoids and subjected them to long-term expansion with the addition of WNT3A or R-spondin1. We confirmed that the organoids have similar genetic profiles, molecular characteristics, and morphological architectures to the corresponding patient tumor tissue during and after long-term expansion. Interestingly, the cellular heterogeneity of organoids is reflected in their differential response to cisplatin or etoposide. We propose to utilize the organoids as small cell lung cancer patient avatar models that would be ideal for investigating the mechanisms underlying tumor recurrence after chemotherapy, and would ultimately help to develop personalized medicine.

引用

页码：1 / 13

页数：13

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