A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean

被引:37
|
作者
Sun, Tianjie [1 ,2 ,3 ]
Ma, Nan [1 ,2 ,3 ]
Wang, Caiqing [1 ,2 ,3 ]
Fan, Huifen [1 ,2 ,3 ]
Wang, Mengxuan [1 ,2 ,3 ]
Zhang, Jie [1 ,2 ,3 ]
Cao, Jinfeng [4 ,5 ]
Wang, Dongmei [1 ,2 ,3 ]
机构
[1] State Key Lab North China Crop Improvement & Regu, Baoding, Peoples R China
[2] Key Lab Hebei Prov Plant Physiol & Mol Pathol, Baoding, Peoples R China
[3] Hebei Agr Univ, Coll Life Sci, Baoding, Peoples R China
[4] Hebei Key Lab Crop Salt Alkali Stress Tolerance E, Cangzhou, Peoples R China
[5] Acad Agr & Forestry Sci, Cangzhou, Peoples R China
来源
关键词
salt stress; VIGS; GmNHX5; CRISPR; Cas9; hairy roots; PLASMA-MEMBRANE; FUNCTIONAL-CHARACTERIZATION; TRANSCRIPTION FACTORS; ANTIPORTER GENE; DROUGHT STRESS; RESPONSES; SOS1; CELL; OVEREXPRESSION; ACCUMULATION;
D O I
10.3389/fpls.2021.638340
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Salt stress caused by soil salinization, is one of the main factors that reduce soybean yield and quality. A large number of genes have been found to be involved in the regulation of salt tolerance. In this study, we characterized a soybean sodium/hydrogen exchanger gene GmNHX5 and revealed its functional mechanism involved in the salt tolerance process in soybean. GmNHX5 responded to salt stress at the transcription level in the salt stress-tolerant soybean plants, but not significantly changed in the salt-sensitive ones. GmNHX5 was located in the Golgi apparatus, and distributed in new leaves and vascular, and was induced by salt treatment. Overexpression of GmNHX5 improved the salt tolerance of hairy roots induced by soybean cotyledons, while the opposite was observed when GmNHX5 was knockout by CRISPR/Cas9. Soybean seedlings overexpressing GmNHX5 also showed an increased expression of GmSOS1, GmSKOR, and GmHKT1, higher K+/Na+ ratio, and higher viability when exposed to salt stress. Our findings provide an effective candidate gene for the cultivation of salt-tolerant germplasm resources and new clues for further understanding of the salt-tolerance mechanism in plants.
引用
收藏
页数:15
相关论文
共 30 条
  • [21] A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress
    Hauser, Felix
    Horie, Tomoaki
    PLANT CELL AND ENVIRONMENT, 2010, 33 (04): : 552 - 565
  • [22] A SNARE-Like Superfamily Protein SbSLSP from the Halophyte Salicornia brachiata Confers Salt and Drought Tolerance by Maintaining Membrane Stability, K+/Na+ Ratio, and Antioxidant Machinery
    Singh, Dinkar
    Yadav, Narendra Singh
    Tiwari, Vivekanand
    Agarwal, Pradeep K.
    Jha, Bhavanath
    FRONTIERS IN PLANT SCIENCE, 2016, 7
  • [23] SODIUM AND POTASSIUM INTERACTIONS IN INCREASING SALT TOLERANCE OF ATRIPLEX-HALIMUS L .2. NA+ AND K+ UPTAKE CHARACTERISTICS
    MOZAFAR, A
    GOODIN, JR
    OERTLI, JJ
    AGRONOMY JOURNAL, 1970, 62 (04) : 481 - &
  • [24] Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance
    Wu, X. X.
    Li, J.
    Wu, X. D.
    Liu, Q.
    Wang, Z. K.
    Liu, S. S.
    Li, S. N.
    Ma, Y. L.
    Sun, J.
    Zhao, L.
    Li, H. Y.
    Li, D. M.
    Li, W. B.
    Su, A. Y.
    GENETICS AND MOLECULAR RESEARCH, 2016, 15 (02)
  • [25] Regulation of Na+/H+ exchangers, Na+/K+ transporters, and lignin biosynthesis genes, along with lignin accumulation, sodium extrusion, and antioxidant defense, confers salt tolerance in alfalfa
    Rahman, Md Atikur
    Woo, Jae Hoon
    Lee, Sang-Hoon
    Park, Hyung Soo
    Kabir, Ahmad Humayan
    Raza, Ali
    El Sabagh, Ayman
    Lee, Ki-Won
    FRONTIERS IN PLANT SCIENCE, 2022, 13
  • [26] Bacillus amyloliquefaciens strain Q1 inoculation enhances salt tolerance of barley seedlings by maintaining the photosynthetic capacity and intracellular Na+/K+ homeostasis
    Liu, Hongjiang
    Amoanimaa-Dede, Hanna
    Zhang, Yanli
    Wu, Xiaojian
    Deng, Fenglin
    Qin, Yuan
    Qiu, Haiping
    Ouyang, Younan
    Wang, Yanli
    Zeng, Fanrong
    PLANT GROWTH REGULATION, 2025, 105 (01) : 111 - 128
  • [27] Exogenous spermidine improves salt tolerance of pecan-grafted seedlings via activating antioxidant system and inhibiting the enhancement of Na+/K+ ratio
    Wu, Zhiwei
    Wang, Junfeng
    Yan, Daoliang
    Yuan, Huwei
    Wang, Yang
    He, Yi
    Wang, Xiaofei
    Li, Zhen
    Mei, Jiaqi
    Hu, Mengyao
    Zhou, Tingting
    Chong, Sun-Li
    Zheng, Bingsong
    ACTA PHYSIOLOGIAE PLANTARUM, 2020, 42 (05)
  • [28] Exogenous spermidine improves salt tolerance of pecan-grafted seedlings via activating antioxidant system and inhibiting the enhancement of Na+/K+ ratio
    Zhiwei Wu
    Junfeng Wang
    Daoliang Yan
    Huwei Yuan
    Yang Wang
    Yi He
    Xiaofei Wang
    Zhen Li
    Jiaqi Mei
    Mengyao Hu
    Tingting Zhou
    Sun-Li Chong
    Bingsong Zheng
    Acta Physiologiae Plantarum, 2020, 42
  • [29] Expression levels of the Na+/K+ transporter OsHKT2;1 and vacuolar Na+/H+ exchanger OsNHX1, Na enrichment, maintaining the photosynthetic abilities and growth performances of indica rice seedlings under salt stress
    Cattarin Theerawitaya
    Rujira Tisarum
    Thapanee Samphumphuang
    Taruhiro Takabe
    Suriyan Cha-um
    Physiology and Molecular Biology of Plants, 2020, 26 : 513 - 523
  • [30] Expression levels of the Na+/K+ transporter OsHKT2;1 and vacuolar Na+/H+ exchanger OsNHX1, Na enrichment, maintaining the photosynthetic abilities and growth performances of indica rice seedlings under salt stress
    Theerawitaya, Cattarin
    Tisarum, Rujira
    Samphumphuang, Thapanee
    Takabe, Taruhiro
    Cha-um, Suriyan
    PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2020, 26 (03) : 513 - 523