Broad and potent cross clade neutralizing antibodies with multiple specificities in the plasma of HIV-1 subtype C infected individuals

被引：6

作者：

Cheedarla, Narayanaiah ^{[1
]}

Precilla, K. Lucia ^{[1
]}

Babu, Hemalatha ^{[1
]}

Vijayan, K. K. Vidya ^{[1
]}

Ashokkumar, Manickam ^{[1
]}

Chandrasekaran, Padmapriyadarsini ^{[1
]}

Kailasam, Nandagopal ^{[2
]}

Sundaramurthi, Jagadish Chandrabose ^{[1
]}

Swaminathan, Soumya ^{[1
]}

Buddolla, Viswanath ^{[3
]}

Vaniambadi, S. Kalyanaraman ^{[4
]}

Ramanathan, V. D. ^{[1
]}

Hanna, Luke Elizabeth ^{[1
]}

机构：

[1] Natl Inst Res TB, HIV AIDS Div, Dept Clin Res, Clin Res, Madras 600031, Tamil Nadu, India

[2] Kilpauk Med Coll & Hosp, ART Ctr, Madras 600010, Tamil Nadu, India

[3] Gachon Univ, Dept Bionanotechnol, San 65, Seongnam Si 461701, Gyeonggi Do, South Korea

[4] Adv Biosci Labs Inc, Rockville, MD 20850 USA

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

B-CELLS; MONOCLONAL-ANTIBODIES; ENV CLONES; VACCINE; DESIGN; RESPONSES; EPITOPES; INSIGHTS; PG9;

D O I：

10.1038/srep46557

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Broadly Cross clade Neutralizing (BCN) antibodies are recognized as potential therapeutic tools and leads for the design of a vaccine that can protect human beings against various clades of Human Immunodeficiency Virus (HIV). In the present study, we screened plasma of 88 HIV-1 infected ART naive individuals for their neutralization potential using a standard panel of 18 pseudoviruses belonging to different subtypes and different levels of neutralization. We identified 12 samples with good breadth of neutralization (neutralized >90% of the viruses). Four of these samples neutralized even the difficult-to-neutralize tier-3 pseudoviruses with great potency (GMT > 600). Analysis of neutralization specificities indicated that four samples had antibodies with multiple epitope binding specificities, viz. CD4-binding site (CD4BS), glycans in the V1/V2 and V3 regions and membrane proximal external region (MPER). Our findings indicate the strong possibility of identifying highly potent bNAbs with known or novel specificities from HIV-1 subtype C infected individuals from India that can be exploited as therapeutic tools or lead molecules for the identification of potential epitopes for design of a protective HIV-1 vaccine.

引用

页数：13

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