Single-cell profiling of the human decidual immune microenvironment in patients with recurrent pregnancy loss

被引：97

作者：

Guo, Chuang ^{[1
]}

Cai, Pengfei ^{[1
]}

Jin, Liying ^{[1
]}

Sha, Qing ^{[1
]}

Yu, Qiaoni ^{[1
]}

Zhang, Wen ^{[1
]}

Jiang, Chen ^{[1
]}

Liu, Qian ^{[1
]}

Zong, Dandan ^{[1
]}

Li, Kun ^{[1
]}

Fang, Jingwen ^{[1
,2
]}

Lu, Fangting ^{[3
]}

Wang, Yanshi ^{[3
]}

Li, Daojing ^{[3
]}

Lin, Jun ^{[1
]}

Li, Lu ^{[1
]}

Zeng, Zhutian ^{[1
]}

Tong, Xianhong ^{[3
]}

Wei, Haiming ^{[1
]}

Qu, Kun ^{[1
,4
,5
]}

机构：

[1] Univ Sci & Technol China, Dept Oncol, Affiliated Hosp 1,Div Mol Med,USTC, Hefei Natl Lab Phys Sci Microscale,Div Life Sci &, Hefei 230027, Anhui, Peoples R China

[2] HanGene Biotech, Xiaoshan Innovat Polis, Hangzhou 311200, Zhejiang, Peoples R China

[3] Univ Sci & Technol China, Affiliated Hosp 1, USTC, Hefei 230021, Anhui, Peoples R China

[4] Univ Sci & Technol China, CAS Key Lab Innate Immun & Chron Dis, CAS Ctr Excellence Mol Cell Sci, Hefei 230027, Anhui, Peoples R China

[5] Univ Sci & Technol China, Sch Data Sci, Hefei 230027, Anhui, Peoples R China

来源：

CELL DISCOVERY | 2021年 / 7卷 / 01期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

NATURAL-KILLER-CELLS; MATERNAL-FETAL INTERFACE; MACROPHAGES; NK; TRANSCRIPTOMICS; DIFFERENTIATION; POLARIZATION; MOLECULES; MONOCYTES; PATTERNS;

D O I：

10.1038/s41421-020-00236-z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Maintaining homeostasis of the decidual immune microenvironment at the maternal-fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and heterogeneity of leukocytes under recurrent miscarriage in human deciduas remains unclear. To address this, we profiled the respective transcriptomes of 18,646 primary human decidual immune cells isolated from patients with recurrent pregnancy loss (RPL) and healthy controls at single-cell resolution. We discovered dramatic differential distributions of immune cell subsets in RPL patients compared with the normal decidual immune microenvironment. Furthermore, we found a subset of decidual natural killer (NK) cells that support embryo growth were diminished in proportion due to abnormal NK cell development in RPL patients. We also elucidated the altered cellular interactions between the decidual immune cell subsets in the microenvironment and those of the immune cells with stromal cells and extravillous trophoblast under disease state. These results provided deeper insights into the RPL decidual immune microenvironment disorder that are potentially applicable to improve the diagnosis and therapeutics of this disease.

引用

页数：15

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