Dual production of poly (3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum

被引：11

作者：

Jo, Sung-Jin ^{[1
]}

Leong, Chean Ring ^{[1
]}

Matsumoto, Ken'ichiro ^{[1
]}

Taguchi, Seiichi ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2009年 / 107卷 / 04期

关键词：

Biotin; Corynebacterium glutamicum; Dual production; Glutamate; Poly(3-hydroxybutyrate); AMINO-ACIDS; CORYNEFORM BACTERIA; POLYHYDROXYALKANOATES; STREPTOMYCES; ENDOTOXIN; ENZYME;

D O I：

10.1016/j.jbiosc.2008.12.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We previously synthesized poly(3-hydroxybutyrate) [P(3HB)] in recombinant Corynebacterium glutamicum, a prominent producer of amino acids. In this study, a two-step cultivation was established for the dual production of glutamate and P(3HB) due to the differences in the optimal concentration of biotin. Glutamate was extracellularly produced first under the biotin-limited condition of 0.3 mu g/L. Production was then shifted to P(3HB) by addition of biotin to a total concentration of 9 mu g/L. The final products obtained were 18 g/L glutamate and 36 wt% of P(3HB). (c) 2008, The Society for Biotechnology, Japan. All rights reserved.

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页码：409 / 411

页数：3

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