Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis

被引：2

作者：

Ye, Youju ^{[1
,2
]}

Han, Xin ^{[1
]}

Rong, Hao ^{[1
]}

Qian, Renjuan ^{[1
,2
]}

Zheng, Jian ^{[2
]}

Ni, Zhouxian ^{[1
]}

Xu, Li'an ^{[1
]}

机构：

[1] Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Forestry Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China

[2] Zhejiang Inst Subtrop Crops, Wenzhou Key Lab Resource Plant Innovat & Utilizat, Wenzhou 325005, Peoples R China

来源：

GENES | 2022年 / 13卷 / 03期

关键词：

Pinus massoniana; Auxin Response Factor (ARF); PmARF6; transgenic plant; AUX/IAA PROTEINS; AUXIN; EXPRESSION; ARF6; FAMILY; GROWTH;

D O I：

10.3390/genes13030469

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Masson pine (Pinus massoniana) is a core industrial tree species that is used for afforestation in southern China. Previous studies have shown that Auxin Response Factors (ARFs) are involved in the growth and development of various species, but the function of ARFs in Masson pine is unclear. In this research, we cloned and identified Masson pine ARF6 cDNA (PmARF6). The results showed that PmARF6 encodes a protein of 681 amino acids that is highly expressed in female flowers. Subcellular analysis showed that the PmARF6 protein occurred predominantly in the nucleus and cytomembrane of Masson pine cells. Compared with wild-type (WT) Arabidopsis, transgenic Arabidopsis plants overexpressing PmARF6 had fewer rosette leaves, and their flower development was slower. These results suggest that overexpression of PmARF6 may inhibit the flower and leaf development of Masson pine and provide new insights into the underlying developmental mechanism.

引用

页数：10

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