Affinity chromatographic methodologies based on immobilized voltage dependent anion channel isoform 1 and application in protein-ligand interaction analysis and bioactive compounds screening from traditional medicine

被引:25
作者
Li, Qian [1 ]
Qiao, Pan [1 ]
Chen, Xiu [1 ]
Wang, Jing [1 ]
Bian, Liujiao [1 ]
Zheng, Xiaohui [1 ]
机构
[1] Northwest Univ, Coll Life Sci, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Xian 710069, Peoples R China
基金
中国国家自然科学基金;
关键词
Membrane protein; Voltage dependent anion channel isoform 1; Protein-ligand interaction; Bioactive compounds screening; Rheum officinale Baill; RHEUM-OFFICINALE BAILL; MEMBRANE-PROTEIN; HUMAN SERUM; NONLINEAR CHROMATOGRAPHY; BINDING; APOPTOSIS; CANCER; PERFORMANCE; EMODIN; CELLS;
D O I
10.1016/j.chroma.2017.03.023
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Voltage dependent anion channel isoform 1 (VDAC-1) serves as an attractive target of anti-cancer drugs by mediating the entry and exit of metabolites between cytoplasm and mitochondria. This work reports on the preparation of a VDAC-1-based bioaffinity chromatographic stationary phase by linking the protein on lecithin modified microspheres. An assay of chromatographic methods including frontal analysis, zonal elution, injection dependent analysis and nonlinear chromatography were utilized to investigate the bindings of ATP, NADH and NADPH to VDAC-1. Electrostatic interactions were found to be main forces during these bindings. The calculated association constants of the three ligands to VDAC-1 showed good agreements between diverse chromatographic methods. Validated application of the stationary phase was performed by screening anti-cancer compounds of Rheum officinale Baill. using high performance affinity chromatography coupled with electrospray ionization-quadrupole time of flight mass spectrometry. Chrysophanol, emodin, rhein, aloe-emodin and catechin were identified as the bioactive components of the herb. These compounds targeted VDAC-1 through Thr207 and the N-terminal region of the protein. Taken together, the current stationary phase was possible to become a promising tool for protein-ligand interaction analysis and anti-cancer drug screening from complex matrices. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:31 / 45
页数:15
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