Impact of Saliva Collection and Processing Methods on Aspartate Aminotransferase, Creatin Kinase and Lactate Dehydrogenase Activities

被引:8
作者
Barranco, Tomas [1 ]
Ceron, Jose J. [1 ]
Lopez-Jornet, Pia [1 ]
Pastor, Josep [2 ]
Carrillo, Jose M. [3 ]
Rubio, Monica [3 ]
Tornel, Pedro L. [4 ]
Cugat, Ramon [5 ]
Tecles, Fernando [1 ]
Tvarijonaviciute, Asta [1 ]
机构
[1] Univ Murcia, Interdisciplinary Lab, Interlab UMU, Campus Excellence Mare Nostrum, Murcia, Spain
[2] Univ Autonoma Barcelona, Dept Med & Cirugia Anim, Barcelona, Spain
[3] Univ CEU Cardenal Herrera, Dept Anim Med & Surg, Edificio Seminario S-N, Valencia 46113, Spain
[4] Univ Hosp Virgen de la Arixaca, Clin Anal, Murcia, Spain
[5] Hosp Quiron, Dept Orthopaed Surg & Traumatol, Placa Alfonso Comin 5-7, Barcelona 08023, Spain
关键词
Saliva; collection; enzyme; DRUGS;
D O I
10.2116/analsci.17N035
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We aimed to investigate the impact of saliva collection and processing methods on AST, CK and LDH. Saliva was collected from 17 healthy participants by a passive drool. Each saliva sample was distributed into 3 aliquots: not treated, centrifuged, and passed through cotton. Centrifugation improved the precision of assays and produced lower values of AST and CK. The use of cotton resulted in decreased levels of LDH. This data stress the importance of the standardization of sample processing to measure enzymes in saliva.
引用
收藏
页码:619 / 622
页数:4
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