3D Microscopic Imaging and Evaluation of Tubular Tissue Architecture

被引:1
作者
Janacek, J. [1 ]
Capek, M. [1 ]
Michalek, J. [1 ]
Karen, P. [1 ]
Kubinova, L. [1 ]
机构
[1] Acad Sci Czech Republ, Inst Physiol, Dept Biomath, CR-14220 Prague 4, Czech Republic
关键词
Confocal microscopy; Capillaries; Brain; Skeletal muscle; Image analysis; SKELETAL-MUSCLE; CONFOCAL MICROSCOPY; CAPILLARIES; NUMBER; RECONSTRUCTION; STEREOLOGY; SHRINKAGE; ALGORITHM; IMAGES; LENGTH;
D O I
10.33549/physiolres.932679
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
3D microscopy and image analysis provide reliable measurements of length, branching, density, tortuosity and orientation of tubular structures in biological samples. We present a survey of methods for analysis of large samples by measurement of local differences in geometrical characteristics. The methods are demonstrated on the structure of the capillary bed in a rat brain.
引用
收藏
页码:S49 / S55
页数:7
相关论文
共 28 条
  • [1] Methods for compensation of the light attenuation with depth of images captured by a confocal microscope
    Capek, Martin
    Janacek, Jiri
    Kubinova, Lucie
    [J]. MICROSCOPY RESEARCH AND TECHNIQUE, 2006, 69 (08) : 624 - 635
  • [2] Volume Reconstruction of Large Tissue Specimens From Serial Physical Sections Using Confocal Microscopy and Correction of Cutting Deformations by Elastic Registration
    Capek, Martin
    Bruza, Petr
    Janacek, Jiri
    Karen, Petr
    Kubinova, Lucie
    Vagnerova, Radomira
    [J]. MICROSCOPY RESEARCH AND TECHNIQUE, 2009, 72 (02) : 110 - 119
  • [3] The estimation error of skeletal muscle capillary supply is significantly reduced by 3D method
    Cebasek, Vita
    Erzen, Ida
    Vyhnal, Ales
    Janacek, Jiri
    Ribaric, Samo
    Kubinova, Lucie
    [J]. MICROVASCULAR RESEARCH, 2010, 79 (01) : 40 - 46
  • [4] Structural and molecular interrogation of intact biological systems
    Chung, Kwanghun
    Wallace, Jenelle
    Kim, Sung-Yon
    Kalyanasundaram, Sandhiya
    Andalman, Aaron S.
    Davidson, Thomas J.
    Mirzabekov, Julie J.
    Zalocusky, Kelly A.
    Mattis, Joanna
    Denisin, Aleksandra K.
    Pak, Sally
    Bernstein, Hannah
    Ramakrishnan, Charu
    Grosenick, Logan
    Gradinaru, Viviana
    Deisseroth, Karl
    [J]. NATURE, 2013, 497 (7449) : 332 - +
  • [5] Diaspro A, 2010, NANOSCOPY AND MULTIDIMENSIONAL OPTICAL FLUORESCENCE MICROSCOPY, P1, DOI 10.1201/9781420078893-f
  • [6] Tissue shrinkage and unbiased stereological estimation of particle number and size
    Dorph-Petersen, KA
    Nyengaard, JR
    Gundersen, HJG
    [J]. JOURNAL OF MICROSCOPY-OXFORD, 2001, 204 : 232 - 246
  • [7] NON-EUCLIDEAN STATISTICS FOR COVARIANCE MATRICES, WITH APPLICATIONS TO DIFFUSION TENSOR IMAGING
    Dryden, Ian L.
    Koloydenko, Alexey
    Zhou, Diwei
    [J]. ANNALS OF APPLIED STATISTICS, 2009, 3 (03) : 1102 - 1123
  • [8] Characterization of the Capillary Network in Skeletal Muscles From 3D Data
    Erzen, I.
    Janacek, J.
    Kubinova, L.
    [J]. PHYSIOLOGICAL RESEARCH, 2011, 60 (01) : 1 - 13
  • [9] THE CONNEULOR - UNBIASED ESTIMATION OF CONNECTIVITY USING PHYSICAL DISSECTORS UNDER PROJECTION
    GUNDERSEN, HJG
    BOYCE, RW
    NYENGAARD, JR
    ODGAARD, A
    [J]. BONE, 1993, 14 (03) : 217 - 222
  • [10] ABERRATIONS IN CONFOCAL FLUORESCENCE MICROSCOPY INDUCED BY MISMATCHES IN REFRACTIVE-INDEX
    HELL, S
    REINER, G
    CREMER, C
    STELZER, EHK
    [J]. JOURNAL OF MICROSCOPY, 1993, 169 : 391 - 405