Mapping seedling resistance to net form of net blotch (Pyrenophora teres f. teres) in barley using detached leaf assay

To develop molecular markers against Pyrenophora teres f. teres in barley, a detached leaf assay was conducted on two DH populations with a set of 11 single conidial lines (SCLs). Out of these, three showed different reactions in the DH population UschixHHOR3073' and two in (PxV)xHHOR9484'. For SCL QLB', a 1r:1s (2=2.78) segregation was observed in the population UschixHHOR3073'. In contrast to this, a continuous variation was observed for the SCL WvB' and d8_4' in the DH population UschixHHOR3073' and for AR' and net1840' in (PxV)xHHOR9484'. With respect to resistance to the SCL QLB', a single major gene was located on chromosome 7H, and for resistance against SCL WvB', two QTLs were detected on chromosome 3H and 7H, and against SCL d8_4', two loci were mapped on chromosome 3HS in UschixHHOR3073'. In the DH population (PxV)xHHOR9484', one locus conferring resistance to the SCL AR' was located on chromosome 3H. For resistance to SCL net1840', two QTLs were mapped on chromosome 4H and 5H.