Characterization of microsatellite loci in the aphid species Metopeurum fuscoviride (Homoptera, Aphididae)

被引:11
作者
Massonnet, B
Leterme, N
Simon, JC
Weisser, WW
机构
[1] Univ Basel, Inst Zool, CH-4051 Basel, Switzerland
[2] INRA, Zool Lab, F-35650 Le Rheu, France
[3] Univ Jena, Inst Okol, D-07743 Jena, Germany
来源
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 02期
关键词
aphid; metapopulation; Metopeurum fuscoviride; microsatellites;
D O I
10.1046/j.1471-8286.2002.00173.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Small distance movements are of great importance for the dynamics of local populations of insects and can indirectly be assessed using molecular markers. We isolated six polymorphic microsatellite loci in the aphid species Metopeurum fuscoviride Stroyan, monophagous on the common Tansy (Tanacetum vulgare L.). Twenty-five to 30 aphids from seven populations were analysed. Between three and 17 alleles were found per locus and expected heterozygosities ranged from 0.14 to 0.79. In total, 114 multilocus genotypes were found in 192 parthenogenetic aphids analysed, indicating that the resolution will be high enough to study differentiation on a small spatial scale.
引用
收藏
页码:127 / 129
页数:3
相关论文
共 14 条
[1]  
[Anonymous], 1998, Primer 3
[2]  
BUDOWLE B, 1991, AM J HUM GENET, V48, P137
[3]  
ELTON CS, 1925, T ENTOMOL SOC LONDON, P289
[4]  
Estoup A, 1996, MICROSATELLITE MARKE, V78352
[5]   Variation in clonal diversity in glasshouse infestations of the aphid, Aphis gossypii Glover in southern France [J].
Fuller, SJ ;
Chavigny, P ;
Lapchin, L ;
Vanlerberghe-Masutti, F .
MOLECULAR ECOLOGY, 1999, 8 (11) :1867-1877
[6]  
HANSKI, 1997, METAPOPULATION BIOL
[7]  
HEIE OE, 1980, FAUNA ENTOMOLOGICA S, P81
[8]   Microsatellite loci for studying clonal mixing, population structure and inbreeding in a social aphid, Pemphigus spyrothecae (Hemiptera: Pemphigidae) [J].
Johnson, PCD ;
Llewellyn, KS ;
Amos, W .
MOLECULAR ECOLOGY, 2000, 9 (09) :1445-1446
[9]   THE RELATIVE IMPORTANCE OF SHORT-RANGE AND LONG-RANGE MOVEMENT OF FLYING APHIDS [J].
LOXDALE, HD ;
HARDIE, J ;
HALBERT, S ;
FOOTTIT, R ;
KIDD, NAC ;
CARTER, CI .
BIOLOGICAL REVIEWS, 1993, 68 (02) :291-311
[10]   Slaves of the environment: the movement of herbivorous insects in relation to their ecology and genotype [J].
Loxdale, HD ;
Lushai, G .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1999, 354 (1388) :1479-1495