High-level expression and characterization of two serine protease inhibitors from Trichinella spiralis

被引：15

作者：

Zhang, Zhaoxia ^{[1
]}

Mao, Yixian ^{[1
]}

Li, Da ^{[1
]}

Zhang, Yvhan ^{[1
]}

Li, Wei ^{[1
]}

Jia, Honglin ^{[2
]}

Zheng, Jun ^{[2
]}

Li, Li ^{[1
]}

Lu, Yixin ^{[1
]}

机构：

[1] Northeast Agr Univ, Coll Vet Med, Key Lab Anim Common Dis Prevent, 59 Mucai St, Harbin 150030, Peoples R China

[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol Michigan State Univ, Maduan St 427, Harbin 150001, Peoples R China

来源：

VETERINARY PARASITOLOGY | 2016年 / 219卷

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

Trichinella spiralis; Serine protease inhibitor; Trypsin; Chymotrypsin; Pepsin; PROTEINASE-INHIBITORS; MOLECULAR-CLONING; ANISAKIS-SIMPLEX; GENE-EXPRESSION; BRUGIA-MALAYI; SERPIN; NEMATODE;

D O I：

10.1016/j.vetpar.2016.02.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Serine protease inhibitors (SPIs) play important roles in tissue homeostasis, cell survival, development, and host defense. So far, SPIs have been identified from various organisms, such as animals, plants, bacteria, poxviruses, and parasites. In this study, two SPIs (Tsp03044 and TspAd5) were identified from the genome of Trichinella spiralis and expressed in Escherichia coli. Sequence analysis revealed that these two SPIs contained essential structural motifs, which were well conserved within the tumor-infiltrating lymphocytes (TIL) and serpin superfamily. Based on protease inhibition assays, the recombinant Tsp03044 showed inhibitory effects on trypsin, alpha-chymotrypsin, and pepsin, while the recombinant TspAd5 could effectively inhibit the activities of alpha-chymotrypsin and pepsin. Both these inhibitors showed activity between 28 and 48 degrees C. The expression levels of the two SPIs were also determined at different developmental stages of the parasite with real-time PCR. Our results indicate that Tsp03044 and TspAd5 are functional serine protease inhibitors. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：34 / 39

页数：6

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