Synthesis, characterization of (Ag-SnO2) nanoparticles and investigation of its antibacterial and anti-biofilm activities

被引:0
作者
Obeizi, Zahra [1 ]
Benbouzid, Houneida [2 ]
Bouarroudj, Tayeb [3 ,4 ]
Benzaid, Chahrazed [5 ]
Djahoudi, Abdelghani [6 ]
机构
[1] Univ Badji Mokhtar, Fac Sci, Dept Biochem, Lab Biochem & Appl Microbiol, Annaba 23000, Algeria
[2] Univ Badji Mokhtar, Fac Sci, Dept Biol, Lab Cellular Toxicol, Annaba 23000, Algeria
[3] Sci & Tech Res Ctr Phys Chem Anal CRAPC, Tipasa 42004, Algeria
[4] Environm Res Ctr CRE, Annaba 23001, Algeria
[5] Univ Badji Mokhtar, Fac Sci, Dept Biochem, Annaba 23000, Algeria
[6] Univ Badji Mokhtar, Fac Med, Dept Pharm, Lab Microbiol, Annaba 23000, Algeria
关键词
Co-precipitation method; Ag-SnO2; nanoparticles; Antibacterial; Anti-biofilm; Minimum inhibitory concentration; DOPED SNO2 NANOPARTICLES; STAPHYLOCOCCUS-AUREUS; BIOGENIC SYNTHESIS; ANTIOXIDANT; MECHANISM; IONS;
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Silver-doped tin oxide (Ag-doped SnO2) nanoparticles (NPs) were manufactured by using chemical co-precipitation method. The synthesized Ag-doped SnO2 nanoparticles were characterized by using X-Ray Diffraction (XRD) analysis, Dynamic Light Scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Ultraviolet-visible (UV-Vis) spectroscopy. The antibacterial activity of the prepared Ag-SnO2 nanoparticles was carried out by agar well diffusion method and determination of minimum inhibitory concentration against both Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) and Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis), anti-biofilm activity was investigated using 96-well microtiter plate method against Staphylococcus aureus and Pseudomonas aeruginosa. XRD Results confirm formation of Ag-doped SnO2 nanoparticles, the average grain size was found to be 23 nm; while DLS analysis indicates an average size around 29 nm. Morphological findings by SEM indicate dense particles with sponge-like microstructure that are uniformly organized irregularly, The EDX analysis confirms the purity of these nanoparticles with peaks of Ag, Sn, and O atoms. SnO2-Ag NPs were exhibited good antibacterial activity against all bacterial strains tested with maximum zone of inhibition of 27 +/- 1.2 mm for S. aureus, the minimum inhibitory concentration values varied between 8 and 128 mu g/ml. The significant percentage of biofilm inhibition was found 73.96% and 71.19% against S. aureus and P. aeruginosa biofilm, respectively. inhibitory
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页码:10 / 17
页数:8
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