Hepatozoon canis infection in a domestic dog in Southern Brazil confirmed by molecular techniques

被引：7

作者：

Lasta, Camila Serina ^{[1
]}

dos Santos, Andrea Pires ^{[2
]}

da Silva Mello, Fabiola Peixoto ^{[3
]}

Lacerda, Luciana de Almeida ^{[2
]}

Messick, Joanne Belle ^{[4
]}

Diaz Gonzalez, Felix Hilario ^{[5
]}

机构：

[1] Univ Fed Rio Grande do Sul, Hosp Clin Vet, BR-91540000 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Vet, BR-91540000 Porto Alegre, RS, Brazil

[3] Pontificia Univ Catolica Rio Grande Sul PUCRS, Hosp Vet, Uruguaiana, RS, Brazil

[4] Purdue Univ, Dept Vet Pathobiol, W Lafayette, IN 47907 USA

[5] Univ Fed Rio Grande do Sul, Fac Vet, BR-91540000 Porto Alegre, RS, Brazil

来源：

CIENCIA RURAL | 2009年 / 39卷 / 07期

关键词：

Hepatozoon canis; hemoparasite; dog; PCR; Brazil; APICOMPLEXA; SEQUENCE; STATE;

D O I：

10.1590/S0103-84782009005000160

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Canine hepatozoonosis is a vector-borne disease caused by the protozoa Hepatozoon spp. There are few case reports in Brazil and the epidemiology, pathogenicity, vectors and molecular characterization is poorly understood. The present study used a canine presenting weight loss, regenerative anemia, neutropenia and hyperglobulinemia. Hepatozoon spp. gamonts were observed in the blood smear by microscopy. DNA was extracted from blood, and the diagnosis was confirmed by PCR assay. The PCR product was purified from gel and cloned. A fragment of 625bp was sequenced and submitted to the GenBank database as Isolate Porto Alegre. Molecular analysis showed a homology of 98-100% with Hepatozoon canis and less than 92% for H. americanum. This study represents the first molecular confirmation that H. canis is present in Southern Brazil.

引用

页码：2135 / 2140

页数：6

共 24 条

[21] SOUZA C, 2001, CIENCIA ANIMAL, V10, P195
[22] MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0
Tamura, Koichiro
Dudley, Joel
Nei, Masatoshi
Kumar, Sudhir
[J]. MOLECULAR BIOLOGY AND EVOLUTION, 2007, 24 (08) : 1596 - 1599
[23] A comprehensive comparison of multiple sequence alignment programs
Thompson, JD
Plewniak, F
Poch, O
[J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (13) : 2682 - 2690
[24] Clinical Hepatozoon canis infection in a dog in Turkey
Voyvoda, H
Pasa, S
Uner, A
[J]. JOURNAL OF SMALL ANIMAL PRACTICE, 2004, 45 (12) : 613 - 617

← 1 2 3 →