Experimental design for gene microarray experiments and differential expression analysis

被引：0
|
作者：
Bobashev, GV [1 ]
Das, S [1 ]
Das, A [1 ]
机构：
[1] RTI, Div Stat Res, Res Triangle Pk, NC USA
来源：
METHODS OF MICROARRAY DATA ANALYSIS II | 2002年
关键词：
genomics; microarray; experimental design; differential expression; normalization; data quality;
D O I：
10.1007/0-306-47598-7_3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Advances in microarray technology open new challenges in data collection, analysis and interpretation, In this review paper, we focus on issues related to obtaining trustworthy normalized data and results of differential expression analysis. In particular, we briefly summarize discussions on sources of biological and technological variation, experimental design, design of arrays, normalization and error models, differential expression and multiple comparison issues. These issues remain major bottlenecks to developing standards and obtaining useful and applicable results of future analysis such as cluster analysis, network modeling, etc.
引用
收藏
页码：23 / 41
页数：19
相关论文
共 50 条
  • [21] Tutorial on microarray gene expression experiments - An introduction
    Repsilber, D
    Mansmann, U
    Brunner, E
    Ziegler, A
    METHODS OF INFORMATION IN MEDICINE, 2005, 44 (03) : 392 - 399
  • [22] The effect of replication on gene expression microarray experiments
    Pavlidis, P
    Li, QH
    Noble, WS
    BIOINFORMATICS, 2003, 19 (13) : 1620 - 1627
  • [23] Microarray and Bioinformatics Analysis of Gene Expression in Experimental Membranous Nephropathy
    Hauser, Peter V.
    Perco, Paul
    Muehlberger, Irmgard
    Pippin, Jeffrey
    Blonski, Mary
    Mayer, Bernd
    Alpers, Charles E.
    Oberbauer, Rainer
    Shankland, Stuart J.
    NEPHRON EXPERIMENTAL NEPHROLOGY, 2009, 112 (02): : E43 - E58
  • [24] Statistical tests for differential expression in cDNA microarray experiments
    Xiangqin Cui
    Gary A Churchill
    Genome Biology, 4
  • [25] Statistical tests for differential expression in cDNA microarray experiments
    Cui, XQ
    Churchill, GA
    GENOME BIOLOGY, 2003, 4 (04)
  • [26] Microarray analysis of differential gene expression in lead-exposed astrocytes
    Bouton, CMLS
    Hossain, MA
    Frelin, LP
    Laterra, J
    Pevsner, J
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2001, 176 (01) : 34 - 53
  • [27] Differential gene expression in the sensitisation and tolerance to olive pollen: microarray analysis
    Aguerri, M.
    Florido, F.
    Mata, M.
    Lopez-Cacho, J.
    Gallardo, S.
    Palomino, P.
    Lahoz, C.
    Cardaba, B.
    ALLERGY, 2010, 65 : 40 - 41
  • [28] Differential gene expression in plasmacytoid dendritic cells identified by microarray analysis
    Hartig, HL
    Hill, S
    Gunn, MD
    FASEB JOURNAL, 2004, 18 (04): : A423 - A423
  • [29] Microarray analysis of differential gene expression in OSCC cell lines.
    Knight, CB
    Richardson, D
    MacDougall, M
    Thornhill, M
    JOURNAL OF BONE AND MINERAL RESEARCH, 2005, 20 (09) : S213 - S213
  • [30] Microarray analysis of differential gene expression in porcine Peyer's patch
    Dvorak, CMT
    Hyland, KA
    Rutherford, MS
    Murtaugh, MP
    FASEB JOURNAL, 2002, 16 (04): : A677 - A677
12345