Adsorption of trypsin on commercial silica gel

被引：9

作者：

Gomez, Jose M. ^{[1
]}

Dolores Romero, Ma ^{[1
]}

Hodaifa, Gassan ^{[1
]}

de la Parra, Elena ^{[1
]}

机构：

[1] Univ Complutense Madrid, Dept Ingn Quim, E-28040 Madrid, Spain

来源：

ENGINEERING IN LIFE SCIENCES | 2009年 / 9卷 / 04期

关键词：

Enzyme; Immobilization kinetic; Silica-gel; Trypsin; CATALYTIC ACTIVITY; IMMOBILIZATION; HYDROLYSIS; ENZYMES; PROTEIN; STABILITY; MEMBRANES; REACTORS;

D O I：

10.1002/elsc.200900018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The immobilization of trypsin onto various commercial silica gels was studied. Silica gels were used directly and characterized by mercuric porosimetry. Agitation rates (100-740 rpm) and particles size (35-75 to 250-500 mu m) of silica gels did not affect the trypsin immobilization capacity. The pore size (3 to 15 nm) is a limiting factor of the trypsin adsorption onto the mesopores structure of silica gels. The adsorption of trypsin was determined as a function of their initial concentration and multilayer formed at high trypsin concentration.

引用

页码：336 / 341

页数：6

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