Photobiomodulation invigorating collagen deposition, proliferating cell nuclear antigen and Ki67 expression during dermal wound repair in mice

被引：25

作者：

Prabhu, Vijendra ^{[1
,2
]}

Rao, Bola Sadashiva Satish ^{[3
,4
]}

Rao, Anuradha Calicut Kini ^{[5
,6
]}

Prasad, Keerthana ^{[7
]}

Mahato, Krishna Kishore ^{[1
]}

机构：

[1] Manipal Acad Higher Educ, Manipal Sch Life Sci, Dept Biophys, Manipal 576104, Karnataka, India

[2] Manipal Acad Higher Educ, Manipal Inst Technol, Dept Biotechnol, Manipal 576104, Karnataka, India

[3] Manipal Acad Higher Educ, Manipal Sch Life Sci, Dept Radiat Biol & Toxicol, Manipal 576104, Karnataka, India

[4] Manipal Acad Higher Educ, Res Directorate, Manipal 576104, Karnataka, India

[5] Manipal Acad Higher Educ, Dept Pathol, Kasturba Med Coll, Manipal 576104, Karnataka, India

[6] Yenepoya, Yenepoya Med Coll, Dept Pathol, Mangalore 575018, Karnataka, India

[7] Manipal Acad Higher Educ, Manipal Sch Informat Sci, Manipal 576104, Karnataka, India

来源：

LASERS IN MEDICAL SCIENCE | 2022年 / 37卷 / 01期

关键词：

Excisional wounds; Wound repair; Photobiomodulation; Proliferating cell nuclear antigen; Ki67; Collagen deposition; NEON LASER IRRADIATION; TYPES I; 660; NM; THERAPY; LIGHT; RED; WAVELENGTHS; MODEL; PHOTOTHERAPY; PATHWAY;

D O I：

10.1007/s10103-020-03202-z

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The present investigation focuses on understanding the role of photobiomodulation in enhancing tissue proliferation. Circular excision wounds of diameter 1.5 cm were created on Swiss albino mice and treated immediately with 2 J/cm(2) and 10 J/cm(2) single exposures of the Helium-Neon laser along with sham-irradiated controls. During different days of healing progression (day 5, day 10, and day 15), the tissue samples upon euthanization of the animals were taken for assessing collagen deposition by Picrosirius red staining and cell proliferation (day 10) by proliferating cell nuclear antigen (PCNA) and Ki67. The positive influence of red light on collagen synthesis was found to be statistically significant on day 10 (P < 0.01) and day 15 (P < 0.05) post-wounding when compared to sham irradiation, as evident from the image analysis of collagen birefringence. Furthermore, a significant rise in PCNA (P < 0.01) and Ki67 (P < 0.05) expression was also recorded in animals exposed to 2 J/cm(2) when compared to sham irradiation and (P < 0.01) compared to the 10 J/cm(2) treated group as evidenced by the microscopy study. The findings of the current investigation have distinctly exhibited the assenting influence of red laser light on excisional wound healing in Swiss albino mice by augmenting cell proliferation and collagen deposition.

引用

页码：171 / 180

页数：10

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