Growth-associated protein 43 promotes thyroid cancer cell lines progression via epithelial-mesenchymal transition

被引：13

作者：

Zheng, Chen ^{[1
]}

Quan, Rui-Da ^{[1
]}

Wu, Cheng-Yong ^{[1
]}

Hu, Jing ^{[1
]}

Lin, Bang-Yi ^{[1
]}

Dong, Xu-Bing ^{[1
]}

Xia, Er-Jie ^{[1
]}

Bhandari, Adheesh ^{[1
]}

Zhang, Xiao-Hua ^{[1
]}

Wang, Ou-Chen ^{[1
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 1, Dept Thyroid & Breast Surg, Wenzhou 325000, Zhejiang, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2019年 / 23卷 / 12期

基金：

中国国家自然科学基金;

关键词：

EMT; GAP43; lymph node metastasis; oncogene; papillary thyroid cancer; PROGNOSTIC-FACTORS; PHOSPHATIDYLINOSITOL; 3-KINASE/AKT; GENETIC ALTERATIONS; FOLLICULAR VARIANT; SPINAL-CORD; CARCINOMA; MUTATIONS; GAP-43; METASTASIS; PATHWAY;

D O I：

10.1111/jcmm.14460

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Thyroid cancer is maintaining at a high incidence level and its carcinogenesis is mainly affected by a complex gene interaction. By analysis of the next-generation resequencing of paired papillary thyroid cancer (PTC) and adjacent thyroid tissues, we found that Growth Associated Protein 43 (GAP43), a phosphoprotein activated by protein kinase C, might be novel markers associated with PTC. However, its function in thyroid carcinoma has been poorly understood. We discovered that GAP43 was significantly overexpressed in thyroid carcinoma and these results were consistent with that in The Cancer Genome Atlas (TCGA) cohort. In addition, some clinicopathological features of GAP43 in TCGA database showed that up-regulated GAP43 is significantly connected to lymph node metastasis (P < 0.001) and tumour size (P = 0.038). In vitro experiments, loss of function experiments was performed to investigate GAP43 in PTC cell lines (TPC-1 and BCPAP). The results proved that GAP43 knockdown in PTC cell significantly decreased the function of cell proliferation, colony formation, migration, and invasion and induced cell apoptosis. Furthermore, we also indicated that GAP43 could modulate the expression of epithelial-mesenchymal transition-related proteins, which could influence invasion and migration. Put those results together, GAP43 is a gene which was associated with PTC and might be a potential therapeutic target.

引用

页码：7974 / 7984

页数：11