Halogenated pesticide transformation by a laccase-mediator system

被引：89

作者：

Torres-Duarte, Cristina ^{[1
]}

Roman, Rosa ^{[1
]}

Tinoco, Raunel ^{[1
]}

Vazquez-Duhalt, Rafael ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico

来源：

CHEMOSPHERE | 2009年 / 77卷 / 05期

关键词：

Laccase; Pesticide adduct; Pesticide transformation; Redox Mediator; ENZYMES; ORGANOPHOSPHORUS; PHENOLS; BIODEGRADATION; DEGRADATION; OXIDATION; ANILINES;

D O I：

10.1016/j.chemosphere.2009.07.039

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The transformation of organic halogenated pesticides by laccase-mediator system has been investigated. Twelve pesticides were assayed in the presence of nine different mediators. Acetosyringone and syringaldehyde showed to be the best mediators. The halogenated pesticides bromoxynil, niclosamide, bromofenoxim and dichlorophen were transformed by the laccase-syringaldehyde system showing catalytic activities of 48.8, 142.0, 166.2 and 1257.6 nmol min(-1) U-1, respectively. The highest pesticide transformation rates were obtained with a mediator-substrate proportion of 5:1, one of the lowest reported so far for the laccase-mediator systems. The analysis of the main product from the dichlorophen transformation showed that an oxidative dehalogenation is involved in the catalytic mechanism. Adduct formation between the mediator syringaldehyde and the pesticides dichlorophen or bromoxynil was also found after enzymatic oxidation. The main goal of this work is to evaluate environmental-friendly mediators for the pesticide transformation, and the potential of laccase-mediator system to efficiently reduce the environmental impact of organic halogenated pesticides is discussed. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：687 / 692

页数：6

共 26 条

[1] Oxidative biodegradation of phosphorothiolates by fungal laccase [J].

Amitai, G ;

Adani, R ;

Sod-Moriah, G ;

Rabinovitz, I ;

Vincze, A ;

Leader, H ;

Chefetz, B ;

Leibovitz-Persky, L ;

Friesem, D ;

Hadar, Y .

FEBS LETTERS, 1998, 438 (03) :195-200

[2]

Atterby H., 2002, Pesticide Outlook, V13, P9, DOI 10.1039/b200937b

[3] Fungal enzymes for environmental purposes, a molecular biology challenge [J].

Ayala, Marcela ;

Pickard, Michael A. ;

Vazquez-Duhalt, Rafael .

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 15 (2-3) :172-180

[4] Promoting laccase activity towards non-phenolic substrates: a mechanistic investigation with some laccase-mediator systems [J].

Baiocco, P ;

Barreca, AM ;

Fabbrini, M ;

Galli, C ;

Gentili, P .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2003, 1 (01) :191-197

[5] Fungal laccases - occurrence and properties [J].

Baldrian, P .

FEMS MICROBIOLOGY REVIEWS, 2006, 30 (02) :215-242

[6] DECONTAMINATING SOIL WITH ENZYMES [J].

BOLLAG, JM .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1992, 26 (10) :1876-1881

[7] Lignin-derived compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes [J].

Camarero, S ;

Ibarra, D ;

Martínez, MJ ;

Martínez, AT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (04) :1775-1784

[8]

Chefetz B, 1998, APPL ENVIRON MICROB, V64, P3175

[9] First evidence of catalytic mediation by phenolic compounds in the laccase-induced oxidation of lignin models [J].

d'Acunzo, F ;

Galli, C .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (17) :3634-3640

[10] Transformation of halogenated pesticides by versatile peroxidase from Bjerkandera adusta [J].

Davila-Vazquez, G ;

Tinoco, R ;

Pickard, MA ;

Vazquez-Duhalt, R .

ENZYME AND MICROBIAL TECHNOLOGY, 2005, 36 (2-3) :223-231

← 1 2 3 →