Optical monitoring of therapeutic drugs with a novel fluorescence-based POCT device

被引：16

作者：

Berrettoni, C. ^{[1
,3
]}

Berneschi, S. ^{[1
]}

Bernini, R. ^{[2
]}

Giannetti, A. ^{[1
]}

Grimaldi, I. A. ^{[2
]}

Persichetti, G. ^{[2
]}

Testa, G. ^{[2
]}

Tombelli, S. ^{[1
]}

Trono, C. ^{[1
]}

Baldini, F. ^{[1
]}

机构：

[1] CNR, Inst Appl Phys Nello Carrara, Via Madonna del Piano 10, I-50019 Sesto Fiorentino, FI, Italy

[2] CNR, Inst Electromagnet Sensing Environm, I-80124 Naples, Italy

[3] Univ Siena, Dept Informat Engn & Math, I-53100 Siena, Italy

来源：

28TH EUROPEAN CONFERENCE ON SOLID-STATE TRANSDUCERS (EUROSENSORS 2014) | 2014年 / 87卷

关键词：

immunosuppressants; tacrolimus; optical biochip; fluorescence anisotropy; TACROLIMUS; BLOOD;

D O I：

10.1016/j.proeng.2014.11.732

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A novel optical biochip for immunosuppressants detection in transplanted patients is described. The optical working of the chip, based on total internal reflection fluorescence and consisting of two bonded polymeric parts, a Zeonex 330 slide and a Topas foil was demonstrated. In parallel, a heterogeneous competitive immunoassay for tacrolimus was implemented using a thirteen-microchannel optical chip interrogated by an in-house optical platform based on fluorescence anisotropy and the bioassay conditions were optimized: a limit of detection of 0.11 ng/mL, a limit of quantification of 0.57 ng/mL and a coefficient of variation of 25% were achieved. (C) 2014 The Authors. Published by Elsevier Ltd.

引用

页码：392 / 395

页数：4

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