Parkinson Phenotype in Aged PINK1-Deficient Mice Is Accompanied by Progressive Mitochondrial Dysfunction in Absence of Neurodegeneration

被引：278

作者：

Gispert, Suzana ^{[1
]}

Ricciardi, Filomena ^{[1
]}

Kurz, Alexander ^{[1
]}

Azizov, Mekhman ^{[1
]}

Hoepken, Hans-Hermann ^{[1
]}

Becker, Dorothea ^{[1
]}

Voos, Wolfgang ^{[1
]}

Leuner, Kristina ^{[1
]}

Mueller, Walter E. ^{[1
]}

Kudin, Alexei P. ^{[1
]}

Kunz, Wolfram S. ^{[1
]}

Zimmermann, Annabelle ^{[1
]}

Roeper, Jochen ^{[1
]}

Wenzel, Dirk ^{[1
]}

Jendrach, Marina ^{[1
]}

Garcia-Arencibia, Moises ^{[1
]}

Fernandez-Ruiz, Javier ^{[1
]}

Huber, Leslie ^{[1
]}

Rohrer, Hermann ^{[1
]}

Barrera, Miguel ^{[1
]}

Reichert, Andreas S. ^{[1
]}

Rueb, Udo ^{[1
]}

Chen, Amy ^{[1
]}

Nussbaum, Robert L. ^{[1
]}

Auburger, Georg ^{[1
]}

机构：

[1] Univ Bonn, Med Ctr, Dept Epileptol, Bonn, Germany

来源：

PLOS ONE | 2009年 / 4卷 / 06期

关键词：

D O I：

10.1371/journal.pone.0005777

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Parkinson's disease (PD) is an adult-onset movement disorder of largely unknown etiology. We have previously shown that loss-of-function mutations of the mitochondrial protein kinase PINK1 (PTEN induced putative kinase 1) cause the recessive PARK6 variant of PD. Methodology/Principal Findings: Now we generated a PINK1 deficient mouse and observed several novel phenotypes: A progressive reduction of weight and of locomotor activity selectively for spontaneous movements occurred at old age. As in PD, abnormal dopamine levels in the aged nigrostriatal projection accompanied the reduced movements. Possibly in line with the PARK6 syndrome but in contrast to sporadic PD, a reduced lifespan, dysfunction of brainstem and sympathetic nerves, visible aggregates of alpha-synuclein within Lewy bodies or nigrostriatal neurodegeneration were not present in aged PINK1-deficient mice. However, we demonstrate PINK1 mutant mice to exhibit a progressive reduction in mitochondrial preprotein import correlating with defects of core mitochondrial functions like ATP-generation and respiration. In contrast to the strong effect of PINK1 on mitochondrial dynamics in Drosophila melanogaster and in spite of reduced expression of fission factor Mtp18, we show reduced fission and increased aggregation of mitochondria only under stress in PINK1-deficient mouse neurons. Conclusion: Thus, aging Pink1(-/-) mice show increasing mitochondrial dysfunction resulting in impaired neural activity similar to PD, in absence of overt neuronal death.

引用

页数：15