Bromelain: an overview of industrial application and purification strategies

被引：157

作者：

Arshad, Zatul Iffah Mohd ^{[1
,2
]}

Amid, Azura ^{[1
]}

Yusof, Faridah ^{[1
]}

Jaswir, Irwandi ^{[1
]}

Ahmad, Kausar ^{[3
]}

Loke, Show Pau ^{[4
]}

机构：

[1] Int Islamic Univ Malaysia, Fac Engn, Dept Biotechnol Engn, Bioproc & Mol Engn Res Unit, Kuala Lumpur 50728, Malaysia

[2] Univ Malaysia Pahang, Fac Chem & Nat Resources Engn, Kuantan 26300, Pahang, Malaysia

[3] Int Islamic Univ Malaysia, Kuliyyah Pharm, Kuala Lumpur 50728, Malaysia

[4] Univ Nottingham, Mfg & Ind Proc Div, Fac Engn, Ctr Food & Bioprod Proc, Semenyih 43500, Selangor Darul, Malaysia

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2014年 / 98卷 / 17期

关键词：

Bromelain; Cysteine protease; Purification; Recombinant bromelain; AMINO-ACID-COMPOSITION; STEM BROMELAIN; ANANAS-COMOSUS; CYSTEINE PROTEINASES; PINEAPPLE-STEM; MOISTURE ENHANCEMENT; FRUIT BROMELAIN; STAIN REMOVAL; EC; 3.4.22.33; WHEAT-FLOUR;

D O I：

10.1007/s00253-014-5889-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.

引用

页码：7283 / 7297

页数：15

共 120 条

[11]

Chaiwut Phanuphong, 2007, Chiang Mai Journal of Science, V34, P109

[12] Efficacy of Extrinsic Stain Removal by Novel Dentifrice Containing Papain and Bromelain Extracts [J].