A rapid method for RNA preparation from Gram-positive bacteria

被引：32

作者：

Oh, ET

So, JS ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[2] Inha Univ, Ctr Adv Bioseparat Technol, Inchon 402751, South Korea

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 52卷 / 03期

关键词：

RNA preparation; glass beads; Gram-positive bacteria;

D O I：

10.1016/S0167-7012(02)00218-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a rapid method for preparation of RNA from various Gram-positive bacteria. Unlike some methods, this method does not require lysozyme and proteinase K. Instead, we used glass beads to break cells more efficiently. Using this method, we successfully isolated the total RNA from various Gram-positive bacteria. This method is rapid, simple, and more economic when compared to the previously reported ones. Prepared RNA can be used for the transcriptional analysis of various Gram-positive bacteria. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：395 / 398

页数：4

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