Sensitive, Specific, and Rapid Detection of Leishmania donovani DNA by Loop-Mediated Isothermal Amplification

被引：65

作者：

Takagi, Hidekazu ^{[1
]}

Itoh, Makoto ^{[1
]}

Islam, Mohammad Zahidul ^{[1
]}

Razzaque, Abdur ^{[2
]}

Ekram, A. R. M. Saifuddin ^{[2
]}

Hashighuchi, Yoshihisa ^{[3
]}

Noiri, Eisei ^{[4
,5
]}

Kimura, Eisaku ^{[1
]}

机构：

[1] Aichi Med Univ, Sch Med, Dept Parasitol, Nagakute, Aichi 4801195, Japan

[2] Rajshahi Med Coll Hosp, Dept Med, Rajshahi, Bangladesh

[3] Kochi Univ, Dept Parasitol, Kochi Med Sch, Nanko Ku, Kochi 780, Japan

[4] Univ Tokyo, Univ Hosp, Dept Nephrol & Endocrinol, Tokyo, Japan

[5] Univ Tokyo, Univ Hosp, Dept Hemodialysis & Apheresis, Tokyo, Japan

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2009年 / 81卷 / 04期

基金：

日本学术振兴会;

关键词：

POLYMERASE-CHAIN-REACTION; AZAR DERMAL LEISHMANIASIS; NESTED PCR ASSAY; VISCERAL LEISHMANIASIS; KALA-AZAR; DIAGNOSIS; BLOOD; SAMPLES;

D O I：

10.4269/ajtmh.2009.09-0145

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We have applied a loop-mediated isothermal amplification (LAMP) technique to detect Leishmania donovani DNA. The LAMP technique detected 1 fg of L. donovani DNA, which was 10-fold more sensitive than a conventional polymerase chain reaction (PCR). All nested PCR-positive blood samples from visceral leishmaniasis patients were positive with the LAMP technique, and DNA samples from L. infantum, L. major, L. mexicana, L. tropica, L. braziliensis, Plasmodium falciparum, and healthy humans were negative with the LAMP technique. The advantages of the LAMP method are its shorter reaction time, a lack of requirement of sophisticated equipment, and visual judgment of positivity based on the turbidity of reaction mixture. Our LAMP technique can be a better alternative to a conventional PCR, especially under field conditions.

引用

页码：578 / 582

页数：5

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