Differential expression of Bcl-2-related proteins in differentiating NT2 cells

被引：11

作者：

Guillemain, I ^{[1
]}

Gaboyard, S ^{[1
]}

Fontès, G ^{[1
]}

Saunier, M ^{[1
]}

Privat, A ^{[1
]}

Patey, G ^{[1
]}

机构：

[1] Ecole Natl Super Chim Montpellier, INSERM, U336, F-34296 Montpellier 05, France

来源：

NEUROREPORT | 2000年 / 11卷 / 07期

关键词：

BAX; Bcl-2; Bcl-x; differentiation; neuron; NTera-2;

D O I：

10.1097/00001756-200005150-00014

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Although the role of Bcl-2-related proteins as regulators of the apoptotic process has been well documented, recent studies suggest that they might also be implicated in neuronal differentiation. We have studied by immunocytochemistry, Western blotting and RT-PCR the expression pattern of Bcl-x(L), Bcl-2 and BAX in the in vitro model of neuronal differentiation constituted by retinoic acid (RA)-treated NTera-2/D1 (NT2/D1) cells. Whereas BAX level did not change significantly during the RA treatment. Bcl-xL level increased markedly during the first week, before returning to basal level during the second week. Bcl-2 expression, undetectable in undifferentiated cells, increased progressively from the first week. From our results, we suggest that, at least in our model, Bcl-2-related proteins might be involved in neuronal differentiation. NeuroReport 11:1421-1425 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：1421 / 1425

页数：5

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