A sweet success for substrate engineering

被引：3

作者：

Griffith, Byron R. ^{[1
]}

Thorson, Jon S. ^{[1
]}

机构：

[1] Univ Wisconsin, Pharmaceut Sci Div, Sch Pharm, Madison, WI 53705 USA

来源：

NATURE CHEMICAL BIOLOGY | 2006年 / 2卷 / 12期

关键词：

D O I：

10.1038/nchembio1206-659

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemically synthesizing complex oligosaccharides remains a significant challenge. Through the addition of hydrophobic appendages to 'unnatural' substrates, some oligosaccharide-forming glycosyltransferases can direct the formation of distinct sugar linkages while maintaining stereoselectivity.

引用

页码：659 / 660

页数：3

共 9 条

[1] High-throughput screening methodology for the directed evolution of glycosyltransferases [J].

Aharoni, Amir ;

Thieme, Karena ;

Chiu, Cecilia P. C. ;

Buchini, Sabrina ;

Lairson, Luke L. ;

Chen, Hongming ;

Strynadka, Natalie C. J. ;

Wakarchuk, Warren W. ;

Withers, Stephen G. .

NATURE METHODS, 2006, 3 (08) :609-614

[2] Synthesis of glycoproteins [J].

Davis, BG .

CHEMICAL REVIEWS, 2002, 102 (02) :579-601

[3] 'Sweetening' natural products via glycorandomization [J].

Griffith, BR ;

Langenhan, JM ;

Thorson, JS .

CURRENT OPINION IN BIOTECHNOLOGY, 2005, 16 (06) :622-630

[4] Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis [J].

Lairson, Luke L. ;

Watts, Andrew G. ;

Wakarchuk, Warren W. ;

Withers, Stephen G. .

NATURE CHEMICAL BIOLOGY, 2006, 2 (12) :724-728

[5] Glycosynthases: Mutant glycosidases for oligosaccharide synthesis [J].

Mackenzie, LF ;

Wang, QP ;

Warren, RAJ ;

Withers, SG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (22) :5583-5584

[6] Enzymatic synthesis of oligosaccharides and neoglycoconjugates [J].

Murata, Takeomi ;

Usui, Taichi .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2006, 70 (05) :1049-1059

[7] Automated synthesis of oligosaccharides as a basis for drug discovery [J].

Seeberger, PH ;

Werz, DB .

NATURE REVIEWS DRUG DISCOVERY, 2005, 4 (09) :751-763

[8] Modifying the regioselectivity of glycosynthase reactions through changes in the acceptor [J].

Stick, RV ;

Stubbs, KA ;

Watts, AG .

AUSTRALIAN JOURNAL OF CHEMISTRY, 2004, 57 (08) :779-786

[9] Exploiting the reversibility of natural product glycosyltransferase-catalyzed reactions [J].

Zhang, Changsheng ;

Griffith, Byron R. ;

Fu, Qiang ;

Albermann, Christoph ;

Fu, Xun ;

Lee, In-Kyoung ;

Li, Lingjun ;

Thorson, Jon S. .

SCIENCE, 2006, 313 (5791) :1291-1294

← 1 →