Modelling Pseudomonas fluorescens and Pseudomonas aeruginosa biofilm formation on stainless steel surfaces and controlling through sanitisers

被引：20

作者：

Rosado Castro, Marcilia Santos ^{[1
]}

Fernandes, Meg da Silva ^{[2
]}

Kabuki, Dirce Yorika ^{[3
]}

Kuaye, Arnaldo Yoshiteru ^{[2
]}

机构：

[1] Fed Inst Southeast Minas Gerais, Div Food, Campus Barbacena,Rua Monsenhor Jose Augusto 204, BR-36205018 Barbacena, MG, Brazil

[2] Univ Campinas UNICAMP, Dept Food Technol, Rua Monteiro Lobato 80,Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP, Brazil

[3] Univ Campinas UNICAMP, Dept Food Sci, Rua Monteiro Lobato 80,Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP, Brazil

来源：

INTERNATIONAL DAIRY JOURNAL | 2021年 / 114卷

关键词：

BACILLUS-CEREUS; PHENOTYPIC CHARACTERIZATION; ENTEROCOCCUS-FAECALIS; CONTAMINATION ROUTES; MILK; TEMPERATURE; PRODUCTS; BACTERIA; RICOTTA; FAECIUM;

D O I：

10.1016/j.idairyj.2020.104945

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Pseudomonas spp. contamination was screened from a Minas Frescal cheese processing plant. Biofilm formation of Pseudomonas fluorescens and Pseudomonas aeruginosa and the effect of three sanitisers, peracetic acid, sodium hypochlorite, and chlorhexidine gluconate, on the biofilm production was also studied. The biofilms were formed in milk according to a central composite rotatable design (CCRD) at 0, 1.2, 4, 6.8, and 8 days and 7, 13, 27, 41, and 47 degrees C. Pseudomonas spp. counts were observed in milking machine, tank, wall, door handle, floor, drain, and cheese. All Pseudomonas spp. isolates showed lipolytic activity and some proteolytic activity. P. fluorescens was able to form biofilms at 9.8-47 degrees C and 0.9-8 days, while P. aeruginosa formed biofilms at 11-47 degrees C and 0.7-8 days. Complete biofilm removal was not observed for all sanitisers studied, showing difficulty in sanitising the surfaces after the biofilm formation. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页数：9

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