Degradation pathways of phenanthrene by Sinorhizobium sp C4

被引:80
作者
Keum, Young-Soo [1 ]
Seo, Jong-Su [1 ]
Hu, Yuting [1 ]
Li, Qing X. [1 ]
机构
[1] Univ Hawaii, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA
基金
美国国家环境保护局;
关键词
D O I
10.1007/s00253-005-0219-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Sinorhizohium sp. C4 was isolated from a polycyclic aromatic hydrocarbon (PAH)-contaminated site in Hilo, HI, USA. This isolate can utilize phenanthrene as a sole carbon source. Sixteen metabolites of phenanthrene were isolated and identified, and the metabolic map was proposed. Degradation of phenanthrene was initiated by dioxygenation on 1,2- and 3,4-C, where the 3,4-dioxygenation was dominant. Subsequent accumulation of 5,6- and 7,8-benzocoumarins confirmed dioxygenation on multiple positions and extradiol cleavage of corresponding diols. The products were further transformed to 1-hydroxy-2-naphthoic acid and 2-hydroxy-1-naphthoic acid then to naphthalene-1,2-diol. In addition to the typical degradation pathways, intradiol cleavage of phenanthrene-3,4-diol was proposed based on the observation of naphthalene- 1,2dicarboxylic acid. Degradation of naphthalene- 1,2-diol proceeded through intradiol cleavage to produce traps-2-carboxycinnamic acid. Phthalic acid, 4,5-dihydroxyphthalic acid, and protocatechuic acid were identified as probable metabolites of traps-2-carboxycinnamic acid, but no trace salicylic acid or its metabolites were found. This is the first detailed study of PAH metabolism by a Sinorhizobium species. The results give a new insight into microbial degradation of PAHs.
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页码:935 / 941
页数:7
相关论文
共 31 条
[1]   Structure of the ring cleavage product of 1-hydroxy-2-naphthoate, an intermediate of the phenanthrene-degradative pathway of Nocardioides sp strain KP7 [J].
Adachi, K ;
Iwabuchi, T ;
Sano, H ;
Harayama, S .
JOURNAL OF BACTERIOLOGY, 1999, 181 (03) :757-763
[2]   Isolation and characterization of symbiotic N-2-fixing Rhizobium meliloti from soils contaminated with aromatic and chloroaromatic hydrocarbons: PAHs and PCBs [J].
Ahmad, D ;
Mehmannavaz, R ;
Damaj, M .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 1997, 39 (01) :33-43
[3]   Characteristics of phenanthrene-degrading bacteria isolated from soils contaminated with polycyclic aromatic hydrocarbons [J].
Aitken, MD ;
Stringfellow, WT ;
Nagel, RD ;
Kazunga, C ;
Chen, SH .
CANADIAN JOURNAL OF MICROBIOLOGY, 1998, 44 (08) :743-752
[4]   Naphthalene degradation and incorporation of naphthalene-derived carbon into biomass by the thermophile Bacillus thermoleovorans [J].
Annweiler, E ;
Richnow, HH ;
Antranikian, G ;
Hebenbrock, S ;
Garms, C ;
Franke, S ;
Francke, W ;
Michaelis, W .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (02) :518-523
[5]   Purification and characterization of a salicylate hydroxylase involved in 1-hydroxy-2-naphthoic acid hydroxylation from the naphthalene and phenanthrene-degrading bacterial strain Pseudomonas putida BS202-P1 [J].
Balashova, NV ;
Stolz, A ;
Knackmuss, HJ ;
Kosheleva, IA ;
Naumov, AV ;
Boronin, AM .
BIODEGRADATION, 2001, 12 (03) :179-188
[6]   Isolation of adherent polycyclic aromatic hydrocarbon (PAH)-degrading bacteria using PAH-sorbing carriers [J].
Bastiaens, L ;
Springael, D ;
Wattiau, P ;
Harms, H ;
deWachter, R ;
Verachtert, H ;
Diels, L .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (05) :1834-1843
[7]   Temporal change in culturable phenanthrene degraders in response to long-term exposure to phenanthrene in a soil column system [J].
Bodour, AA ;
Wang, JM ;
Brusseau, ML ;
Maier, RM .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (10) :888-895
[8]   Biodegradation of polychlorinated biphenyls by rhizobia: A novel finding [J].
Damaj, M ;
Ahmad, D .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (03) :908-915
[9]   Metabolism of anthracene by a Rhodococcus species [J].
Dean-Ross, D ;
Moody, JD ;
Freeman, JP ;
Doerge, DR ;
Cerniglia, CE .
FEMS MICROBIOLOGY LETTERS, 2001, 204 (01) :205-211
[10]   BACTERIAL METABOLISM OF NAPHTHALENE - CONSTRUCTION AND USE OF RECOMBINANT BACTERIA TO STUDY RING CLEAVAGE OF 1,2-DIHYDROXYNAPHTHALENE AND SUBSEQUENT REACTIONS [J].
EATON, RW ;
CHAPMAN, PJ .
JOURNAL OF BACTERIOLOGY, 1992, 174 (23) :7542-7554