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Degradation pathways of phenanthrene by Sinorhizobium sp C4
被引:80
作者:
Keum, Young-Soo
[1
]
Seo, Jong-Su
[1
]
Hu, Yuting
[1
]
Li, Qing X.
[1
]
机构:
[1] Univ Hawaii, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA
基金:
美国国家环境保护局;
关键词:
D O I:
10.1007/s00253-005-0219-z
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
Sinorhizohium sp. C4 was isolated from a polycyclic aromatic hydrocarbon (PAH)-contaminated site in Hilo, HI, USA. This isolate can utilize phenanthrene as a sole carbon source. Sixteen metabolites of phenanthrene were isolated and identified, and the metabolic map was proposed. Degradation of phenanthrene was initiated by dioxygenation on 1,2- and 3,4-C, where the 3,4-dioxygenation was dominant. Subsequent accumulation of 5,6- and 7,8-benzocoumarins confirmed dioxygenation on multiple positions and extradiol cleavage of corresponding diols. The products were further transformed to 1-hydroxy-2-naphthoic acid and 2-hydroxy-1-naphthoic acid then to naphthalene-1,2-diol. In addition to the typical degradation pathways, intradiol cleavage of phenanthrene-3,4-diol was proposed based on the observation of naphthalene- 1,2dicarboxylic acid. Degradation of naphthalene- 1,2-diol proceeded through intradiol cleavage to produce traps-2-carboxycinnamic acid. Phthalic acid, 4,5-dihydroxyphthalic acid, and protocatechuic acid were identified as probable metabolites of traps-2-carboxycinnamic acid, but no trace salicylic acid or its metabolites were found. This is the first detailed study of PAH metabolism by a Sinorhizobium species. The results give a new insight into microbial degradation of PAHs.
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页码:935 / 941
页数:7
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