Two-step purification method of vitellogenin from three teleost fish species:: rainbow trout (Oncorhynchus mykiss), gudgeon (Gobio gobio) and chub (Leuciscus cephalus)

被引：49
作者：
Brion, F
Rogerieux, F
Noury, P
Migeon, B
Flammarion, P
Thybaud, E
Porcher, JM
机构：
[1] INERIS, Ecotoxicol Risk Assessment Unit, F-60550 Verneuil En Halatte, France
[2] Irstea, Lab Ecotoxicol, Lyon, France
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 737卷 / 1-2期
关键词：
purification; Oncorhynchus mykiss; Gobio gobio; Leuciscus cephalus; vitellogenin;
D O I：
10.1016/S0378-4347(99)00406-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A two-step purification protocol was developed to purify rainbow trout (Oncorhynchus mykiss) vitellogenin (Vtg) and was successfully applied to Vtg of chub (Leuciscus cephalus) and gudgeon (Gobio gobio). Capture and intermediate purification were performed by anion-exchange chromatography on a Resource Q column and a polishing step was performed by gel permeation chromatography on Superdex 200 column. This method is a rapid two-step purification procedure that gave a pure solution of Vtg as assessed by silver staining electrophoresis and immunochemical characterisation. (C) 2000 Elsevier Science B.V. All rights reserved.
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收藏
页码：3 / 12
页数：10
相关论文
共 24 条
[1]   Plasma vitellogenin levels during the annual reproductive cycle of the female rainbow trout (Oncorhynchus mykiss): Establishment and validation of an ELISA [J].
Bon, E ;
Barbe, U ;
Rodriguez, JN ;
Cuisset, B ;
Pelissero, C ;
Sumpter, JP ;
LeMenn, F .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1997, 117 (01) :75-84
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   VITELLOGENIN LEVELS IN MALE AND FEMALE RAINBOW-TROUT (SALMO-GAIRDNERI RICHARDSON) AT VARIOUS STAGES OF THE REPRODUCTIVE-CYCLE [J].
COPELAND, PA ;
SUMPTER, JP ;
WALKER, TK ;
CROFT, M .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1986, 83 (02) :487-493
[4]  
DEVLAMING VL, 1980, COMP BIOCHEM PHYS B, V67, P613
[5]  
HARA A, 1980, COMP BIOCH PHYSL B, V65, P144
[6]  
Harries JE, 1997, ENVIRON TOXICOL CHEM, V16, P534, DOI [10.1002/etc.5620160320, 10.1897/1551-5028(1997)016&lt
[7]  
0534:EAIFUK&gt
[8]  
2.3.CO
[9]  
2]
[10]   INDUCTION BY BETA-ESTRADIOL OF VITELLOGENIN IN STRIPED BASS (MORONE-SAXATILIS) - CHARACTERIZATION AND QUANTIFICATION IN PLASMA AND MUCUS [J].
KISHIDA, M ;
ANDERSON, TR ;
SPECKER, JL .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1992, 88 (01) :29-39
123